Genetic diversity of inner quality and SSR association analysis of wild kiwifruit (Actinidia eriantha)
Genetic diversity of inner quality and SSR association analysis of wild kiwifruit (Actinidia eriantha)
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野生猕猴桃内在品质遗传多样性及SSR关联分析
DOI：
10.1016/j.scienta.2019.01.021
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发表时间：
2019-04-05
影响因子：
4.3
通讯作者：
Xu Xiaobiao
中科院分区：
文献类型：
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作者：
Liao Guanglian;Li Zhangyun;Xu Xiaobiao
为了建立果实品质性状与具有特定等位变异的基因型之间的联系，本研究利用145份野生猕猴桃（毛花猕猴桃）种质资源对果实品质进行评价，并利用其SSR标记分析遗传多样性以及主要内在品质的自然变异与SSR标记之间的相关性。结果表明，供试材料的主要内在品质存在丰富的变异，其中可溶性糖含量（SS）的变异系数最大，其次是类胡萝卜素含量（Car）和叶绿素含量（Chi）。通过主成分分析筛选出16份优良的野生毛花猕猴桃种质。SSR标记的遗传多样性分析表明，每对引物检测到的等位基因数为3 - 13个，平均为5.6个等位基因和2.83个有效等位基因，香农信息指数为1.12，奈氏基因多样性指数为0.566。利用40对SSR标记对供试材料的群体结构和连锁不平衡（LD）进行分析，在基因频率为0.05的条件过滤后，部分SSR多态性标记存在显著的连锁不平衡。当P < 0.05时，发现8个标记与可溶性糖含量（SS）、可滴定酸含量（TA）、叶绿素含量（Chl）、干物质含量（DM）和可溶性固形物含量（SSC）等5种品质性状相关。而当P < 0.01时，仅发现标记A193与可溶性糖含量（SS）之间存在显著相关性。本研究挖掘出了与野生毛花猕猴桃果实内在品质相关的相对稳定的标记，并建立了表型与基因型之间的特定等位变异。研究结果可为今后毛花猕猴桃新品种的选育和遗传改良奠定理论基础。
In order to establish the link between fruit quality traits and genotypes with specific allelic variations, one hundred and forty five wild kiwifruit (A. eriantha) germplasm resources were used to evaluate the fruit quality, and their SSR markers were used to analyze the genetic diversity and also the correlation between the natural variation of their main inner qualities and SSR markers in this study. The results showed that there were abundant variations in the main inner quality of the tested materials, among which the variation coefficient of soluble sugar content (SS) was the largest, followed by carotenoid content (Car) and chlorophyll content (Chi). Sixteen excellent wild A. eriantha germplasms were selected by principal component analysis. Genetic diversity analysis of SSR markers showed that the number of alleles detected per pair of primers was 3(similar to)13, with an average of 5.6 alleles and 2.83 effective alleles, the Shannon 's information index was 1.12, and the Ne's gene diversity index was 0.566. The population structure and linkage disequilibrium (LD) of the tested materials were analyzed by 40 pairs of SSR markers, after conditional filtration with gene frequency of 0.05, there was significant linkage disequilibrium in some of the SSR polymorphism markers. When P < 0.05, eight markers were found to be associated with five quality traits such as SS, titratable acid content (TA), Chl, dry matter (DM) and soluble solids content (SSC). While when P < 0.01, only a significant correlation was found between the marker A193 and SS. In this study, the relative stable markers associated with the inner quality of wild A. eriantha fruit were excavated, and specific allelic variations were established between phenotypes and genotypes. The results could lay a theoretical foundation for the breeding and genetic improvement of novel varieties of A. eriantha in the future.