INTERPHOTORECEPTOR RETINOID-BINDING PROTEIN AND ALPHA-TOCOPHEROL PRESERVE THE ISOMERIC AND OXIDATION-STATE OF RETINOL
INTERPHOTORECEPTOR RETINOID-BINDING PROTEIN AND ALPHA-TOCOPHEROL PRESERVE THE ISOMERIC AND OXIDATION-STATE OF RETINOL
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DOI：
10.1111/j.1751-1097.1992.tb02154.x
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发表时间：
1992-08-01
影响因子：
3.3
通讯作者：
CORSON, DW
中科院分区：
文献类型：
--
作者：
CROUCH, RK;HAZARD, ES;CORSON, DW
视黄醇被引入磷脂囊泡或乙醇中的缓冲液时，会迅速分解成多种产物，包括其醛形式的视网膜。发现低浓度的光感受器间类视黄醇结合蛋白可保护视黄醇免受异构化和氧化。在脂质体或乙醇缓冲溶液中加入-生育酚也可以防止分解。这两种药物都不会干扰杆外段制备中9-顺式视网膜色素的成功再生，也不会干扰类维生素a在分离的杆光感受器中恢复敏感性。
Retinol decomposes rapidly into a number of products, including its aldehyde form, retinal, when introduced into buffer in phospholipid vesicles or ethanol. Interphotoreceptor retinoid-binding protein at low concentrations is found to protect retinol from isomerization and oxidation. The addition of alpha-tocopherol to either liposomes or an ethanolic-buffer solution also prevents decomposition. Neither of these agents interferes with the successful regeneration of pigment with 9-cis retinal in rod outer segment preparations or the restoration of sensitivity by retinoids in isolated rod photoreceptors.