Pannexins, a family of gap junction proteins expressed in brain
Pannexins, a family of gap junction proteins expressed in brain
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DOI：
10.1073/pnas.2233464100
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发表时间：
2003-11-11
影响因子：
11.1
通讯作者：
Monyer, H
中科院分区：
文献类型：
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作者：
Bruzzone, R;Hormuzdi, SG;Monyer, H
数据库搜索已经导致了一个蛋白质家族的鉴定，pannexins，它与无脊椎动物和脊椎动物的间隙连接形成蛋白共享一些结构特征。到目前为止，这些蛋白质的功能还不清楚。为了测试泛内联蛋白在脑电通信中的可能性，我们研究了它们的组织分布和功能特性。在这里，我们发现其中两个基因，pannexin 1 （Px1）和Px2，在中枢神经系统中大量表达。在许多神经元细胞群中，包括海马、嗅球、皮层和小脑，这两种泛联蛋白都有共表达，而在大脑的其他区域，如白质，只发现px1阳性细胞。在爪蟾卵母细胞中，Px1而不是Px2形成功能性半通道。两种泛连接蛋白rna的共注射导致具有功能特性的半通道不同于仅由Px1形成的半通道。在成对的卵母细胞中，Px1单独或与W结合可诱导细胞间通道的形成。同源Px1与异质Px1/Px2通道的功能特征以及Px1和Px2在大脑中的不同表达模式表明，pannexins形成具有不同性质的细胞类型特异性间隙连接，可能具有不同的功能。
Database search has led to the identification of a family of proteins, the pannexins, which share some structural features with the gap junction forming proteins of invertebrates and vertebrates. The function of these proteins has remained unclear so far. To test the possibility that pannexins underlie electrical communication in the brain, we have investigated their tissue distribution and functional properties. Here, we show that two of these, genes, pannexin 1 (Px1) and Px2, are abundantly expressed in the CNS. In many neuronal cell populations, including hippocampus, olfactory bulb, cortex and cerebellum, there is coexpression of both pannexins, whereas in other brain regions, e.g., white matter, only Px1-positive cells were found. on expression in Xenopus oocytes, Px1, but not Px2 forms functional hemichannels. Coinjection of both pannexin RNAs results in hemichannels with functional properties that are different from those formed by Px1 only. In paired oocytes, Px1, alone and in combination with W, induces the formation of intercellular channels. The functional characteristics of homomeric Px1 versus heteromeric Px1/Px2 channels and the different expression patterns of Px1 and Px2 in the brain indicate that pannexins form cell type-specific gap junctions with distinct properties that may subserve different functions.