Immunocytochemical localization of androgen-binding protein in the male rat reproductive tract.
Immunocytochemical localization of androgen-binding protein in the male rat reproductive tract.
复制标题
雄性大鼠生殖道中雄激素结合蛋白的免疫细胞化学定位。
作者：
L. Pelliniemi;M. Dym;G. Gunsalus;N. Musto;C. Bardin;D. Fawcett
采用过氧化物酶-抗过氧化物酶技术，采用冷冻切片和光镜技术研究了年轻成年雄性大鼠生殖道中雄激素结合蛋白（ABP）的定位。在精小管中，阳性反应出现在上皮的顶端部分，在精细胞和/或支持细胞中表现明显。ABP以颗粒形式存在于附睾头近端主要上皮细胞的顶端细胞质和隐管上皮细胞中。附睾头远端以及附睾体和尾部的细胞不含ABP，在ABP被吸收的附睾上皮核上细胞质中存在大量被包被的囊泡和多泡体。结果表明，ABP是由流出管上皮细胞和附睾头近端吸收的。
The localization of androgen-binding protein (ABP) in the reproductive tract of young adult male rats was studied with the peroxidase-antiperoxidase technique using frozen sections and light microscopy. Within the seminiferous tubules, a positive reaction was noted in the apical portion of the epithelium, apparently in spermatids and/or Sertoli cells. ABP was localized in granules in the apical cytoplasm of the principal epithelial cells of the proximal part of the caput epididymis and in the epithelial cells of the ductuli efferentes. The cells in the distal part of the caput as well as the corpus and cauda of the epididymis did not contain ABP. Numerous coated vesicles and multivesicular bodies were present in the supranuclear cytoplasm of the epididymal epithelium where ABP was taken up. The results indicate that ABP is taken up from the lumen by epithelial cells of the ductuli efferentes and proximal part of the caput epididymis.