Dynamic patterns of gene expression and regulatory variation in the maize seed coat.
Dynamic patterns of gene expression and regulatory variation in the maize seed coat.
复制标题
玉米种子外套中基因表达和调节性变异的动态模式。
DOI：
10.1186/s12870-023-04078-1
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发表时间：
2023-02-07
影响因子：
5.3
通讯作者：
Tang, Jihua
中科院分区：
文献类型：
--
作者：
Li, Juan;Wang, Liangfa;Wan, Jiong;Dang, Kuntai;Lin, Yuan;Meng, Shujun;Qiu, Xiaoqian;Wang, Qiyue;Zhao, Jiawen;Mu, Liqin;Luo, Hongbing;Ding, Dong;Chen, Zehui;Tang, Jihua
种子大小是影响玉米产量的重要因素，但其分子机制尚不清楚。种皮是玉米籽粒的三大组成部分之一，在一定程度上决定着种子的大小。种皮也与母本相同，是研究杂种优势的理想材料。本研究从授粉后0d至15d连续采集玉米杂交种豫单888及其亲本的自花授粉种子，进行表型分析、细胞学观察和RNA序列分析。表型数据表明，3DAP和8DAP是研究玉米种皮杂种优势的最佳时间点。细胞学观察表明，玉米种皮优势可能是细胞数量与细胞大小协调的结果。此外，RNA-SEQ结果表明，非加性基因在3-8DAP之间发生了显著变化。然而，相加表达的基因数量并没有显著差异。我们的研究结果表明，杂交种的种皮优势是种子膨大和种皮发育过程中不同时间点基因动态变化引起的非加性表达的结果。基因本体论(GO)丰富和京都基因与基因组百科全书(KEGG)途径丰富表明，与DNA复制、细胞周期调控、昼夜节律和代谢产物积累相关的基因对杂种种皮杂种优势有显著贡献。玉米种皮表型分析表明，3DAP和8DAP是种皮杂种优势研究的重要时间点。我们的发现为DNA复制、细胞周期调节、昼夜节律和亲本表达高低的杂交后代的代谢物积累相关基因提供了证据，是杂交种皮优势的主要贡献者。网上版载有补充材料，可在10.1186/s12870-023-04078-1查阅。
Seed size is an important factor contributing to maize yield, but its molecular mechanism remains unclear. The seed coat, which serves as one of the three components of the maize grain, determines seed size to a certain extent. The seed coat also shares the maternal genotype and is an ideal material for studying heterosis. In this study, the self-pollinated seeds of the maize hybrid Yudan888 and its parental lines were continuously collected from 0 day after pollination (DAP) to 15 DAP for phenotyping, cytological observation and RNA-seq. The phenotypic data showed that 3 DAP and 8 DAP are the best time points to study maize seed coat heterosis. Cytological observations indicated that maize seed coat heterosis might be the result of the coordination between cell number and cell size. Furthermore, the RNA-seq results showed that the nonadditive genes changed significantly between 3 and 8 DAP. However, the number of genes expressed additively was not significantly different. Our findings suggest that seed coat heterosis in hybrid is the result of nonadditive expression caused by dynamic changes in genes at different time points during seed expansion and seed coat development. Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment indicated that genes related to DNA replication, cell cycle regulation, circadian rhythms and metabolite accumulation contributed significantly to hybrid seed coat heterosis. Maize seed coat phenotyping allowed us to infer that 3 DAP and 8 DAP are important time points in the study of seed coat heterosis. Our findings provide evidence for genes involved in DNA replication, cell cycle regulation, circadian rhythms and metabolite accumulation in hybrid with high or low parental expression as major contributors to hybrid seed coat heterosis. The online version contains supplementary material available at 10.1186/s12870-023-04078-1.
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影响因子：
11.6
作者：
He, Guangming;Zhu, Xiaopeng;Deng, Xing-Wang
通讯作者：
Deng, Xing-Wang
影响因子：
11.6
作者：
Garcia, D;Fitz Gerald, JN;Berger, F
通讯作者：
Berger, F
影响因子：
64.8
作者：
Huang, Xuehui;Yang, Shihua;Han, Bin
通讯作者：
Han, Bin
影响因子：
4.6
作者：
Hehenberger, Elisabeth;Kradolfer, David;Kohler, Claudia
通讯作者：
Kohler, Claudia
DOI：
10.1073/pnas.0907024106
发表时间：
2009-11-24
影响因子：
11.1
作者：
Adamski, Nikolai M.;Anastasiou, Elena;Lenhard, Michael
通讯作者：
Lenhard, Michael