Functional identification of a novel 14-3-3 epsilon splicing variant suggests dimerization is not necessary for 14-3-3 epsilon to inhibit UV-induced apoptosis
Functional identification of a novel 14-3-3 epsilon splicing variant suggests dimerization is not necessary for 14-3-3 epsilon to inhibit UV-induced apoptosis
复制标题
新型 14-3-3 epsilon 剪接变体的功能鉴定表明，二聚化对于 14-3-3 epsilon 抑制紫外线诱导的细胞凋亡来说不是必需的
DOI：
10.1016/j.bbrc.2010.04.104
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发表时间：
2010-05-28
影响因子：
3.1
通讯作者：
Yu, Long
中科院分区：
文献类型：
--
作者：
Han, Dingding;Ye, Guangming;Yu, Long
14-3-3蛋白作为二聚体起作用，并且已经被鉴定为涉及多种信号传导途径。本文报道了一种新的人14-3-3- 3RSV剪接变异体（14-3- 3RSV）的鉴定，该变异体来源于一个新的外显子1'插入。插入包含一个终止密码子，并导致14-3-3 bp的截短剪接变体。剪接变体从外显子2翻译，并导致N-末端α-螺旋的缺失，这对二聚化至关重要。因此，14-3-3 β sv不能与14-3-3 zeta形成二聚体。然而，在紫外线照射后，14-3-3 β sv也可以支持细胞存活，表明14-3-3 β sv单体足以保护细胞免于凋亡。(C)2010年爱思唯尔公司All rights reserved.
14-3-3 proteins function as a dimer and have been identified to involve in diverse signaling pathways. Here we reported the identification of a novel splicing variant of human 14-3-3 epsilon (14-3-3 epsilon sv), which is derived from a novel exon 1' insertion. The insertion contains a stop codon and leads to a truncated splicing variant of 14-3-3 epsilon. The splicing variant is translated from the exon 2 and results in the deletion of an N-terminal alpha-helix which is crucial for the dimerization. Therefore, the 14-3-3 epsilon sv could not form a dimer with 14-3-3 zeta. However, after UV irradiation 14-3-3 epsilon sv could also support cell survival, suggesting monomer of 14-3-3 epsilon is sufficient to protect cell from apoptosis. (C) 2010 Elsevier Inc. All rights reserved.