Overcoming the blood-brain barrier by Annexin A1-binding peptide to target brain tumours.
Overcoming the blood-brain barrier by Annexin A1-binding peptide to target brain tumours.
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DOI：
10.1038/s41416-020-01066-2
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发表时间：
2020-11
影响因子：
8.8
通讯作者：
Fukuda MN
中科院分区：
文献类型：
--
作者：
Nonaka M;Suzuki-Anekoji M;Nakayama J;Mabashi-Asazuma H;Jarvis DL;Yeh JC;Yamasaki K;Akama TO;Huang CT;Campos AR;Nagaoka M;Sasai T;Kimura-Takagi I;Suwa Y;Yaegashi T;Shibata TK;Sugihara K;Nishizawa-Harada C;Fukuda M;Fukuda MN
膜联蛋白 A1 在肿瘤脉管系统表面特异性表达。静脉注射 IF7 通过膜联蛋白 A1 靶向肿瘤脉管系统。我们测试了 IF7 克服血脑屏障以及静脉注射 IF7C(RR)-SN38 消除小鼠脑肿瘤的假设。 (1) 通过将表达荧光素酶的黑色素瘤 B16 细胞系 B16-Luc 接种到同系 C57BL/6 小鼠的大脑和皮下来生成双肿瘤模型。每天以7.0μmoles/kg静脉内注射IF7C(RR)-SN38，并使用IVIS成像仪通过化学发光评估肿瘤的生长。在免疫功能低下的 SCID 小鼠中使用 C6-Luc 系产生了类似的双肿瘤模型。 (2)将用10％Solutol HS15配制的IF7C(RR)-SN38以2.5μmoles/kg每天静脉注射到两种脑肿瘤小鼠模型中：C57BL/6小鼠中的B16-Luc细胞和裸鼠中的C6-Luc细胞。 (1) 每日注射 IF7C(RR)-SN38 抑制肿瘤生长，无论细胞系或小鼠品系如何。 (2) 每日注射Solutol配制的IF7C(RR)-SN38导致C57BL/6小鼠中B16-Luc脑肿瘤完全消失，而裸鼠C6-Luc中则没有出现这种情况。 IF7C(RR)-SN38 穿过血脑屏障并抑制小鼠模型中脑肿瘤的生长。 Solutol HS15 配制的 IF7C(RR)-SN38 可能促进抗肿瘤免疫反应。
Annexin A1 is expressed specifically on the tumour vasculature surface. Intravenously injected IF7 targets tumour vasculature via annexin A1. We tested the hypothesis that IF7 overcomes the blood–brain barrier and that the intravenously injected IF7C(RR)-SN38 eradicates brain tumours in the mouse. (1) A dual-tumour model was generated by inoculating luciferase-expressing melanoma B16 cell line, B16-Luc, into the brain and under the skin of syngeneic C57BL/6 mice. IF7C(RR)-SN38 was injected intravenously daily at 7.0 μmoles/kg and growth of tumours was assessed by chemiluminescence using an IVIS imager. A similar dual-tumour model was generated with the C6-Luc line in immunocompromised SCID mice. (2) IF7C(RR)-SN38 formulated with 10% Solutol HS15 was injected intravenously daily at 2.5 μmoles/kg into two brain tumour mouse models: B16-Luc cells in C57BL/6 mice, and C6-Luc cells in nude mice. (1) Daily IF7C(RR)-SN38 injection suppressed tumour growth regardless of cell lines or mouse strains. (2) Daily injection of Solutol-formulated IF7C(RR)-SN38 led into complete disappearance of B16-Luc brain tumour in C57BL/6 mice, whereas this did not occur in C6-Luc in nude mice. IF7C(RR)-SN38 crosses the blood–brain barrier and suppresses growth of brain tumours in mouse models. Solutol HS15-formulated IF7C(RR)-SN38 may have promoted an antitumour immune response.
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影响因子：
4.4
作者：
Ernst, S;Lange, C;Rescher, U
通讯作者：
Rescher, U
影响因子：
8.8
作者：
Nosrati, Adi;Tsai, Katy K.;Daud, Adil
通讯作者：
Daud, Adil
影响因子：
45.3
作者：
Ducreux, M;Ychou, M;Rougier, P
通讯作者：
Rougier, P
影响因子：
15.9
作者：
Prados, MD;Lamborn, K;Kuhn, J
通讯作者：
Kuhn, J
DOI：
10.1073/pnas.1114518108
发表时间：
2011-10-18
影响因子：
11.1
作者：
Agemy, Lilach;Friedmann-Morvinski, Dinorah;Ruoslahti, Erkki
通讯作者：
Ruoslahti, Erkki