Identification of a mutation in the para-sodium channel gene of the cattle tick Rhipicephalus microplus associated with resistance to flumethrin but not to cypermethrin
Identification of a mutation in the para-sodium channel gene of the cattle tick Rhipicephalus microplus associated with resistance to flumethrin but not to cypermethrin
复制标题
DOI：
10.1016/j.ijpara.2010.07.006
复制
发表时间：
2010-12-01
影响因子：
4
通讯作者：
Seddon, J. M.
中科院分区：
文献类型：
--
作者：
Jonsson, N. N.;Cutulle, C.;Seddon, J. M.
对钠通道基因的结构域II S4-5连接区中的突变先前已与澳大利亚牛蜱（微小扇头蜱）中的拟除虫菊酯（SP）抗性相关。这是在核苷酸位置190处的C -> A突变，其导致亮氨酸至异亮氨酸氨基酸取代（L 64 I）。在一项调查的15个牛蜱种群与已知的SP抗性状态，来自澳大利亚的昆士兰州和新南威尔士州，有一个很强的关系（r = 0.98）之间的比例蜱携带L 64 I纯合抗性基因型和生存百分比后，暴露于一个歧视浓度的氯氰菊酯的生物测定，如预期的。在氟菊酯抗性蜱中获得的包括结构域II S4-5接头的167 bp区域的序列鉴定了在核苷酸位置214处的G -> T非同义突变，其导致甘氨酸到缬氨酸的取代（G72 V）。G72 V纯合基因型在每个群体中的频率被认为是中度相关的存活率在氟菊酯的判别浓度在幼虫包测试（r = 0.74）。当G72 V纯合子和L 64 I、G72 V杂合子相加时，基因型与氟菊酯抗性的相关性更强（r = 0.93）。这些结果表明，同一基因中的两个突变之间存在相互作用，因此氟菊酯耐药性可能由两个拷贝的G72 V突变或L 64 I和G72 V杂合子赋予。(C)2010年澳大利亚寄生虫学会由爱思唯尔有限公司出版。保留所有权利。
A mutation in the domain II S4-5 linker region of the para-sodium channel gene has been associated previously with synthetic pyrethroid (SP) resistance in the cattle tick (Rhipicephalus microplus) in Australia. This is a C -> A mutation at nucleotide position 190, which results in a leucine to isoleucine amino acid substitution (L64I). In a survey of 15 cattle tick populations with known SP resistance status, sourced from Queensland and New South Wales in Australia, there was a strong relationship (r = 0.98) between the proportion of ticks carrying the L64I homozygous resistant genotype and the survival percentage after exposure to a discriminating concentration of cypermethrin in the bioassay, as expected. However, among populations resistant only to flumethrin, the L64I homozygous genotype was not found. The sequence obtained for a 167 bp region including domain II S4-5 linker in flumethrin-resistant ticks identified a G -> T non-synonymous mutation at nucleotide position 214 that results in a glycine to valine substitution (G72V). The frequency of the G72V homozygous genotype in each population was found to be moderately related to the survival percentage at the discriminating concentration of flumethrin in the larval packet test (r = 0.74). However, a much stronger relationship between genotype and resistance to flumethrin was observed when the heterozygotes of L64I and G72V were added to the G72V homozygotes (r = 0.93). These results suggest that there is an interaction between the two mutations in the same gene, such that flumethrin resistance might be conferred by either two copies of the G72V mutation or by being a L64I and G72V heterozygote. (C) 2010 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.