High-density linkage maps fail to detect any genetic component to sex determination in a Rana temporaria family
High-density linkage maps fail to detect any genetic component to sex determination in a Rana temporaria family
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DOI：
10.1111/jeb.12747
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发表时间：
2016-01-01
影响因子：
2.1
通讯作者：
Perrin, N.
中科院分区：
文献类型：
--
作者：
Brelsford, A.;Rodrigues, N.;Perrin, N.
在不同的种群或家族中，临时蛙的性染色体分化差异显著：在连锁组2 （LG(2)）上，一些雄性在微卫星标记上显示出分化良好的Y单倍型，而另一些雄性在遗传上与雌性无法区分。我们用RADseq标记分析了来自瑞士低地人群的一个家庭，该家庭没有分化性染色体，并且兄弟姐妹分析未能检测到后代的表型性别与亲代单倍型之间的任何关联。在室外条件下，在一个普通的水箱中饲养后代，并在小蛙阶段进行性别鉴定。我们共绘制了2177个snp（1123个在母亲，1054个在父亲），在两个成年非洲爪蟾中恢复了13个连锁群（=染色体对），这些连锁群与热带爪蟾具有强烈的同属性，尽管它们的差异在2000年左右。两性在杂交定位上存在显著差异，杂交在雌性中均匀分布，而在雄性中仅限于染色体末端。在2177个标记中，没有一个与后代性别有显著关联。考虑到我们非常高的分析能力，我们得出结论，性别决定在这个家庭中不是遗传的；哪些因素决定了性别还有待研究。
Sex chromosome differentiation in Rana temporaria varies strikingly among populations or families: whereas some males display well-differentiated Y haplotypes at microsatellite markers on linkage group 2 (LG(2)), others are genetically undistinguishable from females. We analysed with RADseq markers one family from a Swiss lowland population with no differentiated sex chromosomes, and where sibship analyses had failed to detect any association between the phenotypic sex of progeny and parental haplotypes. Offspring were reared in a common tank in outdoor conditions and sexed at the froglet stage. We could map a total of 2177 SNPs (1123 in the mother, 1054 in the father), recovering in both adults 13 linkage groups (= chromosome pairs) that were strongly syntenic to Xenopus tropicalis despite >200My divergence. Sexes differed strikingly in the localization of crossovers, which were uniformly distributed in the female but limited to chromosome ends in the male. None of the 2177 markers showed significant association with offspring sex. Considering the very high power of our analysis, we conclude that sex determination was not genetic in this family; which factors determined sex remain to be investigated.