Crawfish and lobster allergens: identification and structural similarities with other crustacea.
Crawfish and lobster allergens: identification and structural similarities with other crustacea.
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小龙虾和龙虾过敏原：与其他甲壳类动物的识别和结构相似性。
DOI：
10.1159/000234418
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Lehrer,SB
中科院分区：
文献类型：
--
作者：
Halmepuro,L;Salvaggio,JE;Lehrer,SB
用交叉免疫电泳法研究了小龙虾和龙虾提取物中的抗原性和致敏性成分。与兔抗血清的交叉免疫电泳法显示，小龙虾中有23个抗原，龙虾提取物中有17个抗原。在交叉线免疫电泳法中，两种提取物在抗原结构上相互相似，并与其他甲壳类动物相似。用放射免疫吸附试验(RAST)阳性甲壳类敏感受试者的单个或混合血清，交叉放射免疫电泳法(CRIE)显示6种小龙虾和4种龙虾变应原。由于RAST阴性的不敏感受试者的血清也可观察到放射染色，因此用CRIE抑制法检测了IgE结合的特异性。RAST阳性血清与小龙虾或龙虾提取物预先孵育可减少CROE中的放射染色，而使用对照血清时则没有变化。这些结果证实了在海鲜过敏中存在IgE介导的机制，并证明了甲壳类过敏原之间存在许多共同的抗原决定因素。
Antigenic and allergenic components in crawfish and lobster extracts were studied using crossed immunoelectrophoretic techniques. Crossed immunoelectrophoresis with rabbit antisera revealed 23 antigens in crawfish and 17 antigens in lobster extracts. Both extracts exhibited structural similarities in antigens mutually and with other Crustacea in crossed-line immunoelectrophoresis. Crossed radioimmunoelectrophoresis (CRIE) demonstrated 6 crawfish and 4 lobster allergens when individual or pooled sera from radioallergosorbent test (RAST)-positive crustacea-sensitive subjects were used. Since radiostaining was also observed with sera from RAST-negative nonsensitive subjects, specificity of IgE binding was tested using CRIE-inhibition. Preincubation of RAST-positive sera with crawfish or lobster extract decreased radiostaining in CRIE, while no changes occurred when using control sera. These results confirmed the presence of IgE-mediated mechanisms in seafood allergy and demonstrated a number of shared antigenic determinants among Crustacea allergens.