Gene duplication led to divergence of expression patterns, protein-protein interaction patterns and floral development functions of AGL6-like genes in the basal angiosperm Magnolia wufengensis (Magnoliaceae)
Gene duplication led to divergence of expression patterns, protein-protein interaction patterns and floral development functions of AGL6-like genes in the basal angiosperm Magnolia wufengensis (Magnoliaceae)
复制标题
基因重复导致基部被子植物五峰木兰（木兰科）AGL6样基因的表达模式、蛋白质-蛋白质相互作用模式和花发育功能的差异
DOI：
10.1093/treephys/tpz010
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发表时间：
2019-05-01
期刊：
影响因子：
4
通讯作者：
Chen, Faju
中科院分区：
文献类型：
--
作者：
Ma, Jiang;Deng, Shixin;Chen, Faju
MADS-box家族基因在有花植物的生长发育调控中起着重要作用。AGAMOUS-LIKE 6（AGL 6）-like基因是MADS-box家族中最神秘的亚家族之一，其表达模式多变，功能模糊，长期以来一直困扰着研究者。在裸子植物和被子植物中发现了大量的AGL 6同源物。然而，只有少数已被定性，特别是为基础被子植物类群。雾峰木兰（Magnolia wufengensis）是木兰科木本基生被子植物。本研究对M. wufengensis，MawuAGL 6 -1和MawuAGL 6 -2。系统发育分析表明，这两个AGL 6重复序列可能是在木兰科和樟科分化之前通过基因复制而产生的，基因复制后其表达和PPI模式发生了变化。功能分析表明，MawuAGL 6 -1除了具有促进开花的功能外，还可能参与花分生组织的决定，而MawuAGL 6 -2则优先参与花被片形态发生的调控。这些发现进一步加深了我们对AGL 6谱系基因的进化、表达、相互作用和功能的理解，以及AGL 6谱系基因在进化和生物多样性中的意义。
The MADS-box family genes play critical roles in the regulation of growth and development of flowering plants. AGAMOUS-LIKE 6 (AGL6)-like genes are one of the most enigmatic subfamilies of the MADS-box family because of highly variable expression patterns and ambiguous functions, which have long puzzled researchers. A lot of AGL6 homologs have been identified from gymnosperms and angiosperms. However, only a few have been characterized, especially for basal angiosperm taxa. Magnolia wufengensis is a woody basal angiosperm from the family Magnoliaceae. In the current study, the phylogenesis, expression and protein-protein interaction (PPI) patterns, and functions of two AGL6 homologs from M. wufengensis, MawuAGL6-1 and MawuAGL6-2, were analyzed. Phylogenetic analysis indicated that the two AGL6 duplicates may have arisen by gene duplication before the divergence of Magnoliaceae and Lauraceae, with the diversification of their expression and PPI patterns after gene duplication. Functional analysis revealed that, in addition to common functions in accelerating flowering, MawuAGL6-1 might be responsible for flower meristem determinacy, while MawuAGL6-2 is preferentially recruited to regulate tepal morphogenesis. These findings further advance our understanding of the evolution of phylogenesis, expression, interaction and functions of AGL6 lineage genes from basal angiosperms, as well as the entire AGL6 lineage genes, and the significance of AGL6 lineage genes in the evolution and biological diversity.