Identification of genomic differences between the sexes and sex‐specific molecular markers in Monostroma angicava (Ulvophyceae)
Identification of genomic differences between the sexes and sex‐specific molecular markers in Monostroma angicava (Ulvophyceae)
复制标题
DOI：
10.1111/jpy.13128
复制
发表时间：
2021-01
影响因子：
2.9
通讯作者：
Yusuke Horinouchi;T. Togashi
中科院分区：
文献类型：
--
作者：
Yusuke Horinouchi;T. Togashi
关于绿藻性别间的基因组差异，现有的信息很少。这些差异的检测将使性别歧视分子标记的发展成为可能，这对显示出性别差异很小的藻类是有用的。在这项研究中，我们通过下一代测序，鉴定了蓝藻海洋绿藻Monostroma angicava的雄性和雌性特有的DNA序列，这种藻类具有异形单倍体和双倍体的生活周期。荧光原位杂交(FISH)显示，性别特异序列的信号只存在于相应性别的核中，证实了序列的特异性。针对这些序列的性别特异性分子标记成功地区分了配子体的性别，即使在地理上遥远的种群中也是如此，这表明性别特异性序列是普遍存在的。这些结果一致表明，洞穴微藻的雄配子体和雌性配子体在遗传上是不同的，这意味着这种藻类的性别可能是由遗传决定的。
There is little information available regarding genomic differences between sexes in ulvophycean green algae. The detection of these differences will enable the development of sex‐discriminating molecular markers, which are useful for algae showing little apparent difference between sexes. In this study, we identified male‐ and female‐specific DNA sequences in the ulvophycean marine green alga Monostroma angicava, which has a dioicous heteromorphic haplo‐diplontic life cycle, via next‐generation sequencing. Fluorescence in situ hybridization (FISH) showed that signals for the sex‐specific sequences exist only in the nuclei of the corresponding sex, confirming the specificity of the sequences. Sex‐specific molecular markers that targeted these sequences successfully distinguished the sex of gametophytes even in geographically distant populations, indicating that the sex‐specific sequences are universal. These results consistently suggest that male and female gametophytes of M. angicava are genetically different, implying that sex may be determined genetically in this alga.