DEOXYRIBONUCLEIC ACID REPLICATION IN MEIOSIS OF CHLAMYDOMONAS REINHARDI .I. ISOTOPIC TRANSFER EXPERIMENTS WITH A STRAIN PRODUCING 8 ZOOSPORES
DEOXYRIBONUCLEIC ACID REPLICATION IN MEIOSIS OF CHLAMYDOMONAS REINHARDI .I. ISOTOPIC TRANSFER EXPERIMENTS WITH A STRAIN PRODUCING 8 ZOOSPORES
复制标题
DOI：
10.1016/0022-2836(67)90278-1
复制
发表时间：
1967-01-01
影响因子：
5.6
通讯作者：
KATES, JR
中科院分区：
文献类型：
--
作者：
SUEOKA, N;CHIANG, KS;KATES, JR
用~(15)N密度标记结合细胞学观察，研究了莱茵衣藻减数分裂过程中的DNA复制。结果表明：(1)在合子成熟过程中，没有染色体DNA复制，也没有减数分裂，(2)在合子孢子萌发过程中发生减数分裂，第一次减数分裂之前有一次染色体DNA复制，(3)与减数分裂相关的染色体DNA复制是半保守的，(4)在合子萌发末期，每个受精卵产生8个游动孢子，这表明在配子交配到合子萌发的整个过程中，只有一次DNA复制；(5)在受精卵成熟过程中，DNA组分(M带DNA)大量复制并大量出现。
DNA replication in meiosis of Chlamydomonas reinhardi has been studied by 15N density-labeling experiments coupled with cytological observations. It has been shown that (1) during zygotic maturation, there is neither chromosomal DNA replication nor meiotic division; (2) during zygo-spore germination, meiosis occurs, and the first meiotic division is preceded by a single chromosomal DNA replication; (3) the replication of chromosomal DNA associated with meiosis is semiconservative; (4) only hybrid chromosomal DNA molecules are detected at the end of zygote germination, when eight zoospores emerge from each zygote, indicating that only one DNA replication occurs in the entire period between the mating of gametes and the germination of zygotes; (5) a DNA component (M-band DNA) replicates extensively and appears in large quantity during zygote maturation.