Isoflavones enhance the plasma cholesterol-lowering activity of 7S protein in hypercholesterolemic hamsters
Isoflavones enhance the plasma cholesterol-lowering activity of 7S protein in hypercholesterolemic hamsters
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异黄酮增强高胆固醇仓鼠 7S 蛋白的血浆胆固醇降低活性
DOI：
10.1039/c9fo01432b
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发表时间：
2019-11-01
期刊：
影响因子：
6.1
通讯作者：
Chen, Zhen-Yu
中科院分区：
文献类型：
--
作者：
Liu, Yuwei;Yang, Juan;Chen, Zhen-Yu
先前的研究表明，7S蛋白是大豆降低血浆胆固醇活性的活性成分。推测大豆中的异黄酮可以增强7S蛋白的降胆固醇活性。将48只仓鼠分为6组，分别饲喂不含胆固醇的饲料或5种含12.1%7S蛋白和0-15.62 mg g（-1）胆甾酮的高胆固醇饲料。结果表明，在日粮中添加葛根素能剂量依赖性地提高7S蛋白的降胆固醇活性。与高胆固醇对照饮食相比，在基于7S蛋白的饮食中添加胆甾酮显著降低了肝脏胆固醇蓄积12.6- 26.1%。异黄酮还可以促进中性固醇的排泄，并呈剂量依赖性。在饮食中添加异黄酮有利于调节HMG-CoA还原酶的mRNA表达和蛋白质质量。结果表明，葛根素对7S蛋白降胆固醇活性的增强作用是通过抑制胆固醇的吸收和胆固醇的从头合成来实现的。
Previous studies have shown that 7S protein is the active ingredient responsible for the plasma cholesterol-lowering activity of soybean. It is hypothesized that isoflavones in soybean could enhance the blood cholesterol-lowering activity of 7S protein. Forty-eight hamsters were divided into six groups and fed a non-cholesterol diet or one of the five high-cholesterol diets containing 12.1% 7S protein with 0-15.62 mg g(-1) isoflavones. The results showed that addition of isoflavones in diets dose-dependently enhanced the plasma total cholesterol-lowering activity of 7S protein. Addition of isoflavones in 7S protein-based diets significantly reduced hepatic cholesterol accumulation by 12.6-26.1%, compared with the high cholesterol control diet. Isoflavones could also facilitate excretion of neutral sterols in a dose-dependent manner. Supplementation of isoflavones in diets favourably modulated mRNA expression and the protein mass of HMG-CoA reductase. It was concluded that the enhancing effect of isoflavones on the blood cholesterol-lowering activity of 7S protein was mediated by inhibiting the cholesterol absorption and de novo cholesterol synthesis in hypercholesterolemic hamsters.