Cloning of novel repeat-associated small RNAs derived from hairpin precursors in Oryza sativa.
Cloning of novel repeat-associated small RNAs derived from hairpin precursors in Oryza sativa.
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DOI：
10.1111/j.1745-7270.2007.00346.x
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发表时间：
2007-11
影响因子：
3.7
通讯作者：
C. Yao;Botao Zhao;Wei Li-;Yang Li;Wenming Qin;Bing Huang;Youxin Jin
中科院分区：
文献类型：
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作者：
C. Yao;Botao Zhao;Wei Li-;Yang Li;Wenming Qin;Bing Huang;Youxin Jin
植物非编码小RNA包括microRNAs(MiRNAs)、siRNAs(SiRNAs)和反式siRNAs，在调节细胞基因表达方面发挥着重要作用。在这里，我们分离了21个新的内源小RNA分子，从18到24个核苷酸不等，可以映射到111个发夹前体。进一步的分析表明，这些发夹序列大多起源于推测的微型反向重复转座元件，这是一种主要的DNA转座子类型。考虑到miRNA是发夹状前体，而植物内源siRNAs通常位于转座子区域，我们推测我们克隆的小RNA可能代表了siRNAs和miRNAs进化过程中的中间产物。Northern印迹分析表明，其中5个基因在花中的表达明显高于其他组织，表明它们在花序中具有潜在的功能。综上所述，我们的结果丰富了水稻小RNA的数据，为植物小RNA的注释提供了一个有意义的前景。
Plant small non-coding RNAs including microRNAs (miRNAs), small interfering RNAs (siRNAs) and trans-acting siRNAs, play important roles in modulating gene expression in cells. Here we isolated 21 novel endogenous small RNA molecules, ranging from 18 to 24 nucleotides, in Oryza sativa that can be mapped to 111 hairpin precursors. Further analysis indicated that most of these hairpin sequences originated from putative miniature inverted-repeat transposable elements, a major type of DNA transposon. Considering that miRNA is characteristic of hairpin-like precursor and plant endogenous siRNAs are often located at transposon regions, we hypothesized that our cloned small RNAs might represent the intermediate product in the evolutionary process between siRNAs and miRNAs. Northern blot analysis indicated that five of them were much more abundantly expressed in flower compared to other tissues, implying their potential function in inflorescence. In conclusion, our results enrich rice small RNA data and provide a meaningful perspective for small RNA annotation in plants.