Fish protein hydrolysate production from sardine solid waste by crude pepsin enzymatic hydrolysis in a bioreactor coupled to an ultrafiltration unit
Fish protein hydrolysate production from sardine solid waste by crude pepsin enzymatic hydrolysis in a bioreactor coupled to an ultrafiltration unit
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DOI：
10.1016/j.msec.2012.02.013
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发表时间：
2012-05-01
影响因子：
7.9
通讯作者：
Mameri, N.
中科院分区：
文献类型：
--
作者：
Benhabiles, M. S.;Abdi, N.;Mameri, N.
本研究的目的是优化生产鱼蛋白水解物（FPH）的酶水解沙丁鱼固体废物使用粗胃蛋白酶，并放大过程中的生物反应器耦合到超滤单元的产品回收。结果表明，在pH = 1.5 ~ 2、保温时间为6 h的条件下，羊胃粘膜自溶制备的粗胃蛋白酶可用于鱼固体废弃物的酶解。酶促反应的最佳条件为：温度48 ℃，pH 1.5。放大的酶水解和耦合的反应器的超滤单元，以浓缩水解产物得到了良好的结果与蛋白质水解产物的截留系数在90%的范围内。体积浓缩因子为2.5，渗透通量为200 L m（-2）bar（-1）。然而，结果还表明，超滤产物浓缩过程可能超过临界通量运行，在该临界通量处发生不可逆的膜污染。(c)2012爱思唯尔有限公司版权所有。
The aims of the study were to optimize the production a fish protein hydrolysate (FPH) by enzymatic hydrolysis of sardine solid waste using crude pepsin, and to scale up the process in a bioreactor coupled to an ultrafiltration unit for product recovery. Results showed that the crude pepsin prepared by autolysis of the mucous membranes of a sheep stomach at optimal conditions (i.e. pH = 1.5-2 and incubation time of 6 h) could be satisfactory used for the enzymatic hydrolysis of fish solid waste. The optimal conditions for enzymatic reaction were: temperature 48 degrees C, and pH 1.5. The scale up of the enzymatic hydrolysis and the coupling of the reactor an ultrafiltration unit to concentrate the hydrolysate gave good results with a rejection coefficient for the protein hydrolysate product in the range of 90%. The volumetric concentration factor was 2.5, with a permeate flux of 200 L m(-2) bar(-1). However, the results also suggest that the ultrafiltration product concentration process may be operating beyond the critical flux at which point irreversible membrane fouling occurs. (c) 2012 Elsevier B.V. All rights reserved.