TEMPERATURE-DEPENDENT INDUCTION OF THERMOTOLERANCE BY ETHANOL
TEMPERATURE-DEPENDENT INDUCTION OF THERMOTOLERANCE BY ETHANOL
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DOI：
10.2307/3576920
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发表时间：
1986-12-01
影响因子：
3.4
通讯作者：
NAGLE, WA
中科院分区：
文献类型：
--
作者：
HENLE, KJ;MOSS, AJ;NAGLE, WA
乙醇（1 M）对异步中国仓鼠卵巢细胞的细胞毒性具有较强的温度依赖性，细胞存活曲线在34 ~ 39℃之间。C与在不含乙醇的高温培养基中获得的相似。低于36.度。，生存曲线是双相的，表明在乙醇暴露期间耐热性的发展。室温下（22度）。C)乙醇完全无毒，孵育期长达6小时。有乙醇和没有乙醇的生存曲线的比较表明，乙醇的主要作用是有效的温度变化约6.5度。C， 37度时细胞存活曲线。在1 M乙醇中的C等于在43.6.6℃时的C。C在无乙醇的培养基中。除了有效的温度变化外，乙醇还导致对“热”的敏化，其温度依赖性类似于降温加热效应。急性乙醇暴露（25 min, 37℃）诱导耐热性。摄氏或60分钟，35.5度。C)，动力学和耐受性大小与单独加热（10分钟，45度）的同毒调理处理相似。C)。相反，22度的等摩尔乙醇。C不诱导耐热性。这些数据为文献中关于乙醇诱导耐热性的相互矛盾的结果提供了理论依据。热敏化和耐热性的诱导都可以解释为乙醇对细胞内水溶液性质的影响。这些溶剂的改变降低了引起细胞毒性和发展耐热性所必需的温度。
Ethanol (1 M) cytotoxicity in asynchronous Chinese hamster ovary cells was strongly temperature dependent, yielding families of cell survival curves between 34 and 39.degree. C that were similar to those obtained at hyperthermic temperatures in medium without ethanol. Below 36.degree. , survival curves were biphasic, indicating the development of thermotolerance during ethanol exposures. At room temperature (22.degree. C) ethanol was completely nontoxic with incubation periods up to 6 h. A comparison of survival curves with and without ethanol showed that the major effect of ethanol was an effective temperature shift of circa 6.5.degree. C, the cell survival curve at 37.degree. C in 1 M ethanol was equivalent to that at 43.6.degree. C in medium without ethanol. In addition to the effective temperature shift, ethanol also resulted in sensitization to "heat" with a temperature dependence that was similar to the stepdown heating effect. When thermotolerance was induced with acute ethanol exposures (25 min, 37.degree. C or 60 min, 35.5.degree. C), the kinetics and the magnitude of tolerance were similar to those after isotoxic conditioning treatments with heat alone (10 min, 45.degree. C). In contrast, equimolar ethanol at 22.degree. C did not induce thermotolerance. These data provide a rationale for conflicting results in the literature regarding thermotolerance induction by ethanol. Both heat sensitization and the induction of thermotolerance are interpreted as the effect of ethanol on the solution properties of intracellular water. These solvent alterations reduce the temperature necessary to elicit cytotoxicity and the development of thermotolerance.