Podoplanin is expressed in subsets of tumors of the central nervous system
Podoplanin is expressed in subsets of tumors of the central nervous system
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DOI：
10.1007/s00428-005-0133-x
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发表时间：
2006-04-01
期刊：
影响因子：
3.5
通讯作者：
Fukayama, M
中科院分区：
文献类型：
--
作者：
Shibahara, J;Kashima, T;Fukayama, M
用单克隆抗体D2-40进行免疫组织化学分析以确定podoplanin（a.k.a. T1-α、gp 36或aggrus）在中枢神经系统（CNS）肿瘤中的作用，并确定该抗体的诊断效用。对325例不同组织学类型的肿瘤进行了分析。室管膜瘤（37/40，92.5%）、脉络丛乳头状瘤（8/8，100%）和脑膜瘤（100/100，100%）的主要免疫反应性几乎恒定。反应性被认为是“组织特异性的”，因为每个肿瘤的相应正常组织也被发现表达podoplanin。此外，表达，不致力于谱系，被发现在许多其他肿瘤类型，包括星形细胞瘤，髓母细胞瘤，血管母细胞瘤，具有可变的频率和强度。其表达方式尚不完全清楚，但在星形细胞肿瘤中的表达似乎与明显的纤维性质或恶性表型相关，如在毛细胞性星形细胞瘤（12/12，100%）和胶质母细胞瘤（29/35，82.9%）中的高频率表达所示。目前的研究表明，podoplanin表达在几种类型的中枢神经系统肿瘤的频率和强度可变。考虑到podoplanin的广泛表达，抗体D2-40在CNS肿瘤的诊断实践中几乎没有用处。
Immunohistochemical analyses with the monoclonal antibody D2-40 were performed to ascertain the expression of podoplanin (a.k.a. T1-alpha, gp36, or aggrus) in tumors of the central nervous system (CNS) and to determine the diagnostic utility of the antibody. The analyses were performed on 325 tumors of various histologic types. The chief finding was almost constant immunoreactivity in ependymal tumors (37/40, 92.5%), choroid plexus papillomas (8/8, 100%), and meningiomas (100/100, 100%). The reactivity was considered "tissue-specific," as the corresponding normal tissue of each tumor was also found to express podoplanin. In addition, expression, not committed to the lineages, was found in many other tumor types, including astrocytic tumors, medulloblastomas, and hemangioblastomas, with variable frequency and intensity. The way of expression was not fully understood, but the expression in astrocytic tumors seemed to be associated with pronounced fibrous properties or malignant phenotype, as was shown by high-frequent expression in pilocytic astrocytomas (12/12, 100%) and glioblastomas (29/35, 82.9%). The present study has shown that podoplanin is expressed in several types of CNS tumors with variable frequency and intensity. Given the widespread expression of podoplanin, the antibody D2-40 is of little use in diagnostic practice for CNS tumors.