Arabidopsis thaliana plants acclimated to low dose rates of ultraviolet B radiation show specific changes in morphology and gene expression in the absence of stress symptoms
Arabidopsis thaliana plants acclimated to low dose rates of ultraviolet B radiation show specific changes in morphology and gene expression in the absence of stress symptoms
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DOI：
10.1111/j.1469-8137.2007.02092.x
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发表时间：
2007-01-01
期刊：
影响因子：
9.4
通讯作者：
Guisez, Yves
中科院分区：
文献类型：
--
作者：
Hectors, Kathleen;Prinsen, Els;Guisez, Yves
紫外线B(UV-B)驯化包括复杂且鲜为人知的植物新陈代谢变化。通过测定UV-B辐射对植物形态、生理生化和基因表达的影响，研究了UV-B辐射对拟南芥的形态、生理、生化和基因表达的影响，结果表明，UV-B辐射不影响植物的光合作用，也不影响胁迫响应基因的表达，表明UV-B驯化植物不受胁迫。UV诱导的驯化植株的形态变化包括莲座丛直径减小、花序高度减小和开花茎数增加，表明长期UV-B处理引起了植物生长的重新分配，而不是停止生长。基因表达谱表明，UV诱导的形态发生与植物激素(生长素、油菜素类固醇和赤霉素)的动态平衡和细胞壁的细微变化有关。根据基因表达谱的比较，得出结论：适应低、慢性剂量率的UV-B与适应急性、应激诱导的UV-B剂量率不同。因此，UV-B诱导的形态发生在功能上不受胁迫反应的影响。
Ultraviolet B (UV-B) acclimation comprises complex and poorly understood changes in plant metabolism. The effects of chronic and ecologically relevant UV-B dose rates on Arabidopsis thaliana were determined.The UV-B acclimation process was studied by measuring radiation effects on morphology, physiology, biochemistry and gene expression.Chronic UV-B radiation did not affect photosynthesis or the expression of stress responsive genes, which indicated that the UV-acclimated plants were not stressed. UV-induced morphological changes in acclimated plants included decreased rosette diameter, decreased inflorescence height and increased numbers of flowering stems, indicating that chronic UV-B treatment caused a redistribution rather than a cessation of growth. Gene expression profiling indicated that UV-induced morphogenesis was associated with subtle changes in phytohormone (auxins, brassinosteroids and gibberellins) homeostasis and the cell wall.Based on the comparison of gene expression profiles, it is concluded that acclimation to low, chronic dose rates of UV-B is distinct from that to acute, stress-inducing UV-B dose rates. Hence, UV-B-induced morphogenesis is functionally uncoupled from stress responses.