Transgenic monkeys produced by retroviral gene transfer into mature oocytes
Transgenic monkeys produced by retroviral gene transfer into mature oocytes
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DOI：
10.1126/science.291.5502.309
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发表时间：
2001-01-12
期刊：
影响因子：
56.9
通讯作者：
Schatten, G
中科院分区：
文献类型：
--
作者：
Chan, AWS;Chong, KY;Schatten, G
将复制缺陷型逆转录病毒载体注射到224个恒河猴成熟卵母细胞的卵黄周隙中，获得了携带绿色荧光蛋白（GFP）基因的转基因恒河猴。从20个胚胎移植出生的三个男性中，一个是转基因的，当可访问的组织进行检测转基因DNA和信使RNA。从73天流产的异卵双胞胎中研究的所有组织携带转基因，如Southern分析所证实的，并且通过直接和间接荧光成像检测GFP转基因报告基因。
Transgenic rhesus monkeys carrying the green fluorescent protein (GFP) gene were produced by injecting pseudotyped replication-defective retroviral vector into the perivitelline space of 224 mature rhesus oocytes, Later fertilized by intracytoplasmic sperm injection. Of the three males born from 20 embryo transfers, one was transgenic when accessible tissues were assayed for transgene DNA and messenger RNA. ALL tissues that were studied from a fraternal set of twins, miscarried at 73 days, carried the transgene, as confirmed by Southern analyses, and the GFP transgene reporter was detected by both direct and indirect fluorescence imaging.