Quantitative T-cell interferon-gamma responses to Mycobacterium tuberculosis-specific antigens in active and latent tuberculosis
Quantitative T-cell interferon-gamma responses to Mycobacterium tuberculosis-specific antigens in active and latent tuberculosis
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DOI：
10.1007/s10096-008-0670-8
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发表时间：
2009-06-01
影响因子：
4.5
通讯作者：
Wang, Y. T.
中科院分区：
文献类型：
--
作者：
Chee, C. B. E.;Barkham, T. M. S.;Wang, Y. T.
目的是比较在活动性和潜伏性结核病（TB）状态下通过商业干扰素-γ（IFN-γ）释放测定（IGRA）测量的定量T细胞应答。将培养证实的结核病例的T细胞应答与IGRA结果阳性的接触者和结核菌素皮肤试验阳性的接触者进行了比较。270例活动性结核病例和183例社区接触者的T-SPOT.TB结果显示，高于阴性对照/2.5 × 105外周血单核细胞的中值斑点形成细胞（SFC）为27（-1至203）对10（-2至174）响应于ESAT-6（p < 0.001）;和37（0至293）对13（0至225）响应于CFP-10（p < 0.001）。在270例病例和142名聚集性接触者中，通过QuantiFERON-TB Gold In-tube测量的中位IFN γ水平（抗原减去零对照）为2.3 IU/ml（-0.58至31.44）vs 1.7 IU/ml（0.35至26.51，p = 0.98）。通过T-SPOT.TB测量的定量T细胞应答可指示分枝杆菌负荷和疾病活动性，但不能用于区分活动性和潜伏性TB。
The objective was to compare the quantitative T-cell responses measured by the commercial interferon-gamma (IFN gamma) release assays (IGRAs) in active and latent tuberculosis (TB) states. T-cell responses of culture-proven TB cases were compared with those of contacts with positive IGRA results and tuberculin skin tests a parts per thousand yen 15 mm. T-SPOT.TB results in 270 active TB cases and 183 community contacts showed the median spot-forming cells (SFCs) above negative control/2.5 x 10(5) peripheral blood mononuclear cells to be 27 (-1 to 203) vs 10 (-2 to 174) in response to ESAT-6 (p < 0.001); and 37 (0 to 293) vs 13 (0 to 225) to CFP-10 (p < 0.001). The median IFN gamma levels (antigen minus nil control) as measured by QuantiFERON-TB Gold In-tube in 270 cases and 142 contacts in congregate settings was 2.3 IU/ml (-0.58 to 31.44) vs 1.7 IU/ml (0.35 to 26.51, p = 0.98). Quantitative T-cell responses as measured by the T-SPOT.TB may indicate mycobacterial burden and disease activity, but cannot be used to discriminate active from latent TB.