EPICARDIAL FORMATION IN EMBRYONIC CHICK HEART - COMPUTER-AIDED RECONSTRUCTION, SCANNING, AND TRANSMISSION ELECTRON-MICROSCOPIC STUDIES
EPICARDIAL FORMATION IN EMBRYONIC CHICK HEART - COMPUTER-AIDED RECONSTRUCTION, SCANNING, AND TRANSMISSION ELECTRON-MICROSCOPIC STUDIES
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DOI：
10.1002/aja.1001840204
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发表时间：
1989-02-01
影响因子：
--
通讯作者：
HIRAKOW, R
中科院分区：
文献类型：
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作者：
HIRUMA, T;HIRAKOW, R
通过基于用于光学显微镜的连续树脂切片的计算机辅助重建，并利用扫描电子显微镜和透射电子显微镜进行进一步详细观察，揭示了鸡胚心脏从初始阶段到最终阶段的心外膜形成过程。心外膜起源于23对体节（第14 +期）时静脉窦外表面右侧的间皮细胞簇突起。这些突起伸长形成若干绒毛状突起，在30对体节（第17期）时其末端最终接触到胚胎心脏的背壁。间皮细胞从这些黏附部位开始，向各个方向逐渐扩散到心肌细胞上，形成单层片状覆盖物。因此，到第23期时，心室完全被心外膜覆盖，并且在心外膜下层内出现血岛样结构。直到第25期心房才被心外膜包裹，而在第27期，不断推进的心外膜到达动脉球的最远端。文中还给出了鸡心脏心外膜形成的时间表。
Epicardial formation in the embryonic chick heart from initial to final stages was revealed by means of computer-aided reconstructions based on serial resin sections for light microscopy, with further detailed observations using scanning and transmission electron microscopy. The origin of the epicardium was recognized as protrusions of mesothelial cell clusters on the right side of the external surface of the sinus venosus at 23 somites (stage 14+). These protrusions elongated to give rise to several villous processes, the tips of which eventually touched the dorsal wall of the embryonic heart at 30 somites (stage 17). Originating from these adhesion sites, mesothelial cells spread gradually onto myocardial cells in all directions to form a monolayered sheetlike cover. Thus, by stage 23, the ventricle was completely overlaid with epicardium, and blood-island-like structures appeared within the subepicardial layer. The atrium was not enveloped by epicardium until stage 25, and the exteme distal end of the bulbus cordis was reached by the advancing epicardium at stage 27. A chronological table of epicardial formation in the chick heart is presented.