Elevated expression of urotensin II and its receptor in diethylnitrosamine-mediated precancerous lesions in rat liver
Elevated expression of urotensin II and its receptor in diethylnitrosamine-mediated precancerous lesions in rat liver
复制标题
DOI：
10.1016/j.peptides.2010.10.032
复制
发表时间：
2011-02-01
期刊：
影响因子：
3
通讯作者：
Wang, Xuejiang
中科院分区：
文献类型：
--
作者：
Wang, Hongxia;Dong, Kun;Wang, Xuejiang
尾加压素II（UII）是一种生长抑素样肽，参与细胞增殖和肿瘤生物学。为探讨肝源性UII在大鼠肝癌前病变发病中的作用，本研究观察了UII及其受体UT在二乙基亚硝胺（DEN）诱导的大鼠肝癌前病变中的表达，以及UII对肝卵圆细胞增殖的影响。采用放射免疫分析、RT-PCR、免疫组化和western blot等方法进行研究。与未处理对照组相比，DEN处理组大鼠血浆中UII含量增加47.7%，肝组织中UII含量增加164.9%（均P
Urotensin II (UII) is a somatostatin-like peptide involved in cell proliferation and in tumor biology. To explore the role of liver-derived UII in the pathogenesis of precancerous liver lesions in rat, we investigated the expression of UII and its receptor, UT, in diethylnitrosamine (DEN)-induced precancerous liver lesions and the effects of UII on cell proliferation by hepatic oval cells. Radioimmunoassay, RT-PCR, immunohistochemistry and western blot were used in this study. Compared with untreated controls, rats treated with DEN showed increased UII content by 47.7% in plasma and by 164.9% in liver tissue (all P