Gold-embedded photosensitive liposomes for drug delivery: Triggering mechanism and intracellular release
Gold-embedded photosensitive liposomes for drug delivery: Triggering mechanism and intracellular release
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DOI：
10.1016/j.jconrel.2010.07.095
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发表时间：
2010-10-01
影响因子：
10.8
通讯作者：
Yliperttula, Marjo
中科院分区：
文献类型：
--
作者：
Paasonen, Lauri;Sipila, Tuomas;Yliperttula, Marjo
包埋金纳米颗粒的脂质体表现出光触发的内容物释放。我们研究了光诱导变化的机制和光诱导细胞释放的功能。实时小角X射线散射(SAXS)分析表明，双硬脂酰磷脂酰胆碱(DSPC)/二棕榈酰磷脂酰胆碱(DPPC)脂质体在受热时发生了随时间变化的相变。载金纳米粒脂质体在紫外光照射下也发生了类似的变化：金纳米粒吸收光能并将其转化为热，从而导致脂质相从凝胶相到波纹相，再到流体相。在没有紫外光照射的情况下，金纳米粒子不影响脂质体的双层周期。通过荧光激活的细胞分选实验，证实了脂质体内化进入ARPE-19细胞后，光触发的亲水性荧光探针(钙黄绿素)从金纳米粒脂质体中释放出来。脂质体制剂在体外不会降低细胞存活率。总而言之，光触发的脂质体释放在细胞中是起作用的，并且释放是由脂双层中的热相变化触发的。(C)2010爱思唯尔B.V.保留所有权利。
Liposomes embedded with gold nanoparticles show light-triggered contents release. We investigated the mechanism of the light-induced changes and functionality of the light-induced release in the cells. The real time small angle X-ray scattering (SAXS) analysis revealed time-dependent phase transitions in distearoylphosphatidylcholine (DSPC)/dipalmitoylphosphatidylcholine (DPPC) liposomes upon heating. Similar changes were observed when gold nanoparticle-embedded liposomes were exposed to the UV light: gold nanoparticles absorb light energy and transfer it to heat, thereby causing lipid phase transition from gel phase to rippled phase, and further to fluid phase. Without UV light exposure the gold nanoparticles did not affect the liposomal bilayer periodicity. The light-triggered release of hydrophilic fluorescent probe (calcein) from the gold nanoparticle-loaded liposomes was demonstrated with fluorescence-activated cell sorting after liposome internalization into the ARPE-19 cells. The liposome formulations did not decrease the cell viability in vitro. In conclusion, the light-triggered release from the liposomes is functional in the cells, and the release is triggered by thermal phase changes in the lipid bilayers. (C) 2010 Elsevier B.V. All rights reserved.