Transparent Intracellular Sensing Platform with Si Needles for Simultaneous Live Imaging
Transparent Intracellular Sensing Platform with Si Needles for Simultaneous Live Imaging
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DOI：
10.1021/acsnano.3c07527
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发表时间：
2023-12-07
期刊：
影响因子：
17.1
通讯作者：
Lee，Chi Hwan
中科院分区：
文献类型：
--
作者：
Park，Woohyun;Kim，Eun Mi;Lee，Chi Hwan
垂直有序的硅针是特别感兴趣的长期细胞内记录，由于其渗透活细胞的能力，可以忽略不计的损害和最小的毒性。这种细胞内记录可以大大受益于同时活细胞成像，而不会破坏它们的培养，有助于深入了解细胞功能和活动。然而，标准的实时成像技术，如倒置和共焦显微镜的使用，目前受到硅晶片的不透明性的阻碍，通常用于制造垂直硅针。在这里，我们介绍了一个透明的细胞内传感平台，它结合了垂直的硅针与一个透明的弹性基板上的金银纳米线的网状结构。该传感平台满足同时进行细胞内记录和成像的所有先决条件，包括电化学阻抗、光学透明度、机械顺应性和细胞活力。该传感平台的概念验证演示包括监测心肌细胞和三维工程心血管组织中的电位，同时使用倒置和共聚焦显微镜进行实时成像。该传感平台在神经科学、心脏病学、肌肉生理学和药物筛选等各个学科的细胞内研究中具有广泛的潜在应用。
Vertically ordered Si needles are of particular interest for long-term intracellular recording owing to their capacity to infiltrate living cells with negligible damage and minimal toxicity. Such intracellular recordings could greatly benefit from simultaneous live cell imaging without disrupting their culture, contributing to an in-depth understanding of cellular function and activity. However, the use of standard live imaging techniques, such as inverted and confocal microscopy, is currently impeded by the opacity of Si wafers, typically employed for fabricating vertical Si needles. Here, we introduce a transparent intracellular sensing platform that combines vertical Si needles with a percolated network of Au–Ag nanowires on a transparent elastomeric substrate. This sensing platform meets all prerequisites for simultaneous intracellular recording and imaging, including electrochemical impedance, optical transparency, mechanical compliance, and cell viability. Proof-of-concept demonstrations of this sensing platform include monitoring electrical potentials in cardiomyocyte cells and in three-dimensionally engineered cardiovascular tissue, all while conducting live imaging with inverted and confocal microscopes. This sensing platform holds wide-ranging potential applications for intracellular research across various disciplines such as neuroscience, cardiology, muscle physiology, and drug screening.