5-Fluorouracil incorporation in DNA of human breast carcinoma cells.
5-Fluorouracil incorporation in DNA of human breast carcinoma cells.
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DOI：
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发表时间：
1982-08
期刊：
影响因子：
11.2
通讯作者：
P. Major;E. Egan;D. Herrick;D. Kufe
中科院分区：
文献类型：
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作者：
P. Major;E. Egan;D. Herrick;D. Kufe
我们以前已经证明了L1210 DNA中存在5-氟尿嘧啶(Fura)残基。这些发现已经扩展到MCF-7人乳腺癌细胞系。用硫酸铯梯度离心法分离MCF-7RNA和DNA组分。碱和核糖核酸酶的消化也被用来去除任何可能污染DNA组分的RNA。纯化的DNA在消化成核苷酸和核苷后，用高压液相色谱进行分析。结果表明，在这些人乳腺癌细胞的DNA中，暴露于5-氟脱氧尿苷的Fura后，在DNA中可以检测到Fura残基。此外，Fura在MCF-7RNA和DNA中的掺入程度与氟化嘧啶相似。我们还证明，通过与胸腺嘧啶核苷同时孵育，可以增强该人类细胞系DNA中的Fura掺入。
We have demonstrated previously the presence of 5-fluorouracil (FUra) residues in L1210 DNA. These findings have been extended to the MCF-7 human breast carcinoma cell line. Cesium sulfate gradient centrifugation has been used to separate the MCF-7 RNA and DNA fractions. Alkali and RNase digests have also been used to remove any possible RNA contaminating the DNA fraction. The purified DNA has been analyzed by high-pressure liquid chromatography following digestion to nucleotides and nucleosides. The results demonstrate that FUra residues are detectable in the DNA of these human breast carcinoma cells following exposure to either FUra of 5-fluorodeoxyuridine. Further, the extend of FUra incorporation in both MCF-7 RNA and DNA is similar with either fluorinated pyrimidine. We also demonstrate that the FUra incorporation in DNA from this human cell line can be enhanced by concurrent incubation with thymidine.