A unique DNA methylation signature defines a population of IFN-γ/IL-4 double-positive T cells during helminth infection.
A unique DNA methylation signature defines a population of IFN-γ/IL-4 double-positive T cells during helminth infection.
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DOI：
10.1002/eji.201344098
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发表时间：
2014-06
影响因子：
5.4
通讯作者：
MacDonald, Andrew S.
中科院分区：
文献类型：
--
作者：
Deaton, Aimee M.;Cook, Peter C.;De Sousa, Dina;Phythian-Adams, Alexander T.;Bird, Adrian;MacDonald, Andrew S.
Th 1和Th 2细胞的命运传统上被认为是相互排斥的，但最近的工作表明，这些谱系可能比以前认为的更具可塑性。当从感染曼氏血吸虫寄生蠕虫的小鼠中分离脾脏CD 4 + T细胞时，我们观察到了确定的IFN-γ/IL-4双阳性细胞群。与IFN-γ + IL-4−（Th 1）和IFN-γ−IL-4+（Th 2）细胞相比，这些IFN-γ+IL-4+细胞在Ifng和Il 4位点的DNA甲基化存在差异，表明它们代表了不同的效应细胞群。IFN-γ+IL-4+细胞还在Gata 3基因体内的CpG岛上显示出离散的DNA甲基化模式，Gata 3基因编码Th 2身份的主调节因子。该区域的DNA甲基化与Gata 3水平降低相关，表明可能在控制Gata 3表达中起作用。这些数据提供了重要的洞察背后的Th 1和Th 2特征共存的分子机制。
Th1 and Th2 cell fates are traditionally viewed as mutually exclusive, but recent work suggests that these lineages may be more plastic than previously thought. When isolating splenic CD4+ T cells from mice infected with the parasitic helminth Schistosoma mansoni, we observed a defined population of IFN-γ/IL-4 double-positive cells. These IFN-γ+IL-4+ cells showed differences in DNA methylation at the Ifng and Il4 loci when compared with IFN-γ+IL-4− (Th1) and IFN-γ−IL-4+ (Th2) cells, demonstrating that they represent a distinct effector cell population. IFN-γ+IL-4+ cells also displayed a discrete DNA methylation pattern at a CpG island within the body of the Gata3 gene, which encodes the master regulator of Th2 identity. DNA methylation at this region correlated with decreased Gata3 levels, suggesting a possible role in controlling Gata3 expression. These data provide important insight into the molecular mechanisms behind the co-existence of Th1 and Th2 characteristics.
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影响因子：
4.4
作者：
Panzer, Marc;Sitte, Selina;Voehringer, David
通讯作者：
Voehringer, David
DOI：
10.1084/jem.20100734
发表时间：
2010-09-27
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Phythian-Adams AT;Cook PC;Lundie RJ;Jones LH;Smith KA;Barr TA;Hochweller K;Anderton SM;Hämmerling GJ;Maizels RM;MacDonald AS
通讯作者：
MacDonald AS
影响因子：
16.8
作者：
Lorincz, MC;Dickerson, DR;Groudine, M
通讯作者：
Groudine, M
影响因子：
32.4
作者：
Hegazy, Ahmed N.;Peine, Michael;Loehning, Max
通讯作者：
Loehning, Max
影响因子：
9.8
作者：
Dessein, AJ;Hillaire, D;Abel, L
通讯作者：
Abel, L