9-Diazomethylanthracene as a new fluorescence and ultraviolet label for the spectrometric detection of picomole quantities of fatty acids by high-pressure liquid chromatography.
9-Diazomethylanthracene as a new fluorescence and ultraviolet label for the spectrometric detection of picomole quantities of fatty acids by high-pressure liquid chromatography.
复制标题
9-重氮甲基蒽作为一种新的荧光和紫外标记，用于通过高压液相色谱法光谱检测皮摩尔量的脂肪酸。
DOI：
10.1016/0003-2697(80)90500-x
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发表时间：
1980
影响因子：
2.9
通讯作者：
Richard D. Morin
中科院分区：
文献类型：
--
作者：
Steven A. Barker;John A. Monti;Samuel T. Christian;F. Benington;Richard D. Morin
9-Diazomethylanthracene reacts with carboxyl groups to give an ester derivative which can be used as either a fluorescence or ultraviolet label for fatty acid analysis by high-pressure liquid chromatography. The limit of detection by ultraviolet spectroscopy was demonstrated to be approximately 150 pg/μl of the individual fatty acid esters. Fluorescence detection showed a limit of approximately 15 pg/μl. The fluorescence detector response was linear from 0.49 to 14.2 pmol/μl. Thus, derivatization of fatty acids with 9-diazomethylanthracene provides a new and very sensitive method for the quantification of picomole quantities of fatty acids by high-pressure liquid chromatographic techniques using either ultraviolet or fluorescence detection.
