Generation of nine induced pluripotent stem cell lines as an ethnic diversity panel
Generation of nine induced pluripotent stem cell lines as an ethnic diversity panel
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DOI：
10.1016/j.scr.2018.07.013
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发表时间：
2018-08-01
影响因子：
1.2
通讯作者：
Sprando, Robert L.
中科院分区：
文献类型：
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作者：
Gao, Xiugong;Yourick, Jeffrey J.;Sprando, Robert L.
人类诱导多能干细胞（iPSC）提供了来自具有特定遗传背景的个体的分化细胞的潜在无限来源。使用自我复制RNA重编程技术，我们从三种不同种族的血液样本来源的内皮祖细胞（EPCs）产生了九个iPSC系：黑人或非裔美国人、拉丁裔或西班牙裔以及非西班牙裔白色。所得到的iPSC系在很大程度上显示出正常的核型，表达多能性标记基因，并在体外自发分化成三个胚层。这些iPSC系提供了产生具有种族多样性的组织的潜力，从而为种族相关毒理学应用提供了有价值的工具。
Human induced pluripotent stem cells (iPSCs) provide a potentially unlimited source of differentiated cells from individuals with specific genetic backgrounds. Using self-replicative RNA reprogramming technology, we generated nine iPSC lines from endothelial progenitor cells (EPCs) derived from blood samples of three different ethnicities: Black or African American, Latino or Hispanic, and Non-Hispanic White. The resulting iPSC lines showed normal karyotype in large part, expressed pluripotency marker genes, and spontaneously differentiated in vitro into the three germ layers. These iPSC lines offer the potential to generate tissues with ethnic diversity, and thus afford a valuable tool for ethnic-related toxicological applications.