Protocols in molecular parasitology.
Protocols in molecular parasitology.
复制标题
分子寄生虫学协议。
作者：
J. E. Hyde
布氏体的培养与生物克隆。克氏体的培养与生物克隆。利什曼原虫体外培养及生物克隆。适于大规模制剂的恶性疟原虫（红细胞期）的简单体外培养。疟原虫的同步与克隆。恶性疟原虫配子体的培养和制备用于免疫化学、分子和蚊子感染性研究。曼陀罗菌的培养与生命周期阶段的生产。布氏体DNA和RNA的制备。克氏锥虫RNA和DNA的分离。利什曼原虫DNA和RNA的分离。恶性疟原虫体外培养DNA和RNA的提取与纯化。血吸虫核酸的提取与纯化。应用DNA和整个生物体过滤支持DNA探针分析：点，槽，和触摸印迹。重复序列DNA探针在寄生虫检测和物种鉴定中的发展和应用。寄生原生动物DNA的流式细胞术分析。PCR低水平检测血液中疟原虫。点突变和基因重排鉴定的PCR方法。疟原虫野外分离株的DNA抗原分型研究。核糖体RNA探针用于物种的检测和鉴定。寄生虫rRNA的快速测序。表达库的抗体筛选。表达克隆鉴定的抗体选择程序。利用合成寡核苷酸分离寄生虫基因。寄生原生动物的染色体定位方法。电穿孔法转染利什曼原虫和布氏锥虫。二维聚丙烯酰胺凝胶电泳。同工酶电泳用于寄生虫鉴定。血吸虫的表面标记。单克隆抗体亲和层析。免疫印迹和酶联免疫吸附试验。寄生虫的免疫荧光。疟疾寄生虫抗原的免疫电镜定位。蛋白质微测序印迹膜的制备。t细胞表位的鉴定。索引。
Culturing and Biological Cloning of T. brucei. Culturing and Biological Cloning of T. cruzi. In Vitro Cultivation and Biological Cloning of Leishmania. Simple In Vitro Cultivation of the Malaria Parasite P. falciparum (Erythrocytic Stages) Suitable for Large-Scale Preparations. Synchronization and Cloning of Malaria Parasites. The Culture and Prparation of Gametocytes of Plasmodium falciparum for Immunochemical, Molecular, and Mosquito Infectivity Studies. The Culture of S. mansoni and Production of Life Cycle Stages. Preparation of DNA and RNA from T. brucei. Isolation of RNA and DNA from T. cruzi. Isolation of DNA and RNA from Leishmania. The Extraction and Purification of DNA and RNA from In Vitro Cultures of the Malaria Parasite P. falciparum. Extraction and Purificaiton of Nucleic Acids from Schistosomes. Application of DNA and Whole Organisms to Filter Supports for DNA Probe Analysis: Dot, Slot, and Touch Blotting. The Development and Use of Repetitive Sequences as DNA Probes for Parasite Detection and Species Identification. Analysis of the DNA of Parasitic Protozoa by Flow Cytometry. PCR for Low-Level Detection of Malaria Parasites in Blood. PCR Methods for Identification of Point Mutations and Gene Rearrangements. Antigenic Typing of Field Isolates of Plasmodium by DNA Techniques. Ribosomal RNA Probes for Detection and Identification of Species. Rapid Sequencing of Parasite rRNA. Antibody Screening of Expression Libraries. Antibody Select Procedure for Characterization of Expression Clones. Isolation of Parasite Genes Using Synthetic Oligonucleotides. Chromosome Mapping Methods for Parasitic Protozoa. Transfection of Leishmania and Trypanosoma brucei by Electroporation. Two-Dimensional Polyacrylamide Gel Electrophoresis. Isoenzyme Electrophoresis for Parasiste Characterization. The Surface Labeling of Schistosomes. Monoclonal Antibody Affinity Chromatography. Immunoblotting and Enzyme Linked Immunosorbent Assay. Immunofluorescence of Parasites. Immunoelectron Microscopic Localization of Antigens in Malaria Parasites. Preparation of Blotted Membrane for Protein Microsequencing. Identification of T-Cell Epitopes. Index.