Vitamin E and organoselenium prevent the cocarcinogenic activity of arsenite with solar UVR in mouse skin.
Vitamin E and organoselenium prevent the cocarcinogenic activity of arsenite with solar UVR in mouse skin.
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维生素 E 和有机硒可防止亚砷酸盐与太阳紫外线对小鼠皮肤的致癌活性。
DOI：
10.1093/carcin/bgi180
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发表时间：
2005
期刊：
影响因子：
4.7
通讯作者：
Rossman,TobyG
中科院分区：
文献类型：
--
作者：
Uddin,AhmedN;Burns,FredricJ;Rossman,TobyG
砷致癌是一个世界性的问题，目前的控制手段有限。最近，我们发现饮用水中的亚砷酸盐大大增强了太阳紫外线辐射（UVR）诱导的小鼠皮肤癌，浓度低至1.25 mg/l。在这项研究中，我们研究了维生素E和1，4-亚苯基双（亚甲基）硒氰酸酯（p-XSC）对UVR和UVR +亚砷酸盐诱导的肿瘤的保护作用。将无毛小鼠暴露于单独的UVR（1.0 kJ/m2×每周3次）或UVR +亚砷酸钠（5 mg/l饮用水），并喂食补充或不补充维生素E（RRR-α-生育酚乙酸酯，62.5 IU/kg饲料）或p-XSC（10 mg/kg）的实验室饲料26周。单独接受UVR的小鼠的肿瘤产量为3.6个肿瘤/小鼠，并且在饮用水中添加亚砷酸盐将产量增加至7.0个肿瘤/小鼠（P< 0.005）。维生素E和p-XSC分别使给予UVR +亚砷酸盐的小鼠的肿瘤产量降低了2.1倍（P< 0.001）和2倍（P< 0.002）。维生素E，而不是p-XSC，使UVR单独诱导的肿瘤产量降低30%（P< 0.05）。在用或不用化学预防剂治疗的小鼠中没有观察到肿瘤类型或恶性程度的显著差异。小鼠皮肤的8-氧代-2 '-脱氧鸟苷（8-氧代-dG）免疫染色显示，与接受UVR +亚砷酸盐处理的小鼠相比，接受维生素E或p-XSC处理的小鼠中8-氧代-dG的形成显着减少。这些结果表明，维生素E和p-XSC强烈地防止亚砷酸盐诱导的UVR致癌作用的增强。
Arsenic-induced carcinogenesis is a worldwide problem for which there is currently limited means for control. Recently, we showed that arsenite in drinking water greatly potentiates solar ultraviolet radiation (UVR) induced skin cancer in mice, at concentrations as low as 1.25 mg/l. In this study, we examined the protective efficacy of vitamin E and 1,4-phenylenebis(methylene)selenocyanate (p-XSC) against tumors induced by UVR and UVR + arsenite. Hairless mice were exposed to UVR alone (1.0 kJ/m2× 3 times weekly) or UVR + sodium arsenite (5 mg/l in drinking water) and fed lab chow supplemented or not with vitamin E (RRR-α-tocopheryl acetate, 62.5 IU/kg diet) orp-XSC (10 mg/kg) for 26 weeks. The tumor yield for mice receiving UVR alone was 3.6 tumors/mouse and the addition of arsenite to the drinking water increased the yield to 7.0 tumors/mouse (P< 0.005). Vitamin E andp-XSC reduced the tumor yield in mice given UVR + arsenite by 2.1-fold (P< 0.001) and 2-fold (P< 0.002), respectively. Vitamin E, but not p-XSC, reduced the tumor yield induced by UVR alone by 30% (P< 0.05). No significant difference in tumor types or grade of malignancy was observed in mice treated with or without chemopreventives. Immunostaining of mouse skin for 8-oxo-2′-deoxyguanosine (8-oxo-dG) revealed a significant reduction of 8-oxo-dG formation in mice treated with vitamin E orp-XSC compared with those treated with UVR + arsenite. These results show that vitamin E andp-XSC protect strongly against arsenite-induced enhancement of UVR carcinogenesis.