Tobacco smoke reduces viability in human lung fibroblasts: protective effect of glutathione S-transferase P1
Tobacco smoke reduces viability in human lung fibroblasts: protective effect of glutathione S-transferase P1
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DOI：
10.1152/ajplung.2001.280.6.l1189
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发表时间：
2001-06-01
影响因子：
4.9
通讯作者：
Ouchi, Y
中科院分区：
文献类型：
--
作者：
Ishii, T;Matsuse, T;Ouchi, Y
吸烟被认为是各种肺部疾病的主要危险因素，包括肺癌和肺气肿。然而，香烟烟雾对肺源性细胞活力的直接影响尚未完全阐明。在这项研究中，我们调查了人肺成纤维细胞衍生（HFL 1）细胞的生存能力，以不同浓度的香烟烟雾提取物（CSE）。CSE在较低浓度（10-25%）下诱导细胞凋亡，在较高浓度（50-100%）下诱导坏死。我们还研究了谷胱甘肽S-转移酶P1（GSTP 1），在肺中的异生物质代谢和抗氧化酶之一，对CSE的细胞毒性的影响。我们的研究结果表明，HFL 1细胞死亡的水平降低了转染GST 1表达载体和GST 1反义载体转染增加。因此，GST 1的瞬时过表达和低表达似乎抑制和增强CSE对HFL 1细胞的细胞毒性作用，这表明GST 1可能对气道细胞中的香烟烟雾具有保护作用。
Cigarette smoking is thought to be a major risk factor in various lung diseases including lung cancer and emphysema. However, the direct effect of cigarette smoke on the viability of lung-derived cells has not been fully elucidated. In this study, we investigated the viability of human lung fibroblast-derived (HFL1) cells to different concentrations of cigarette smoke extract (CSE). CSE induced apoptosis at lower concentrations (10-25%) and necrosis at higher concentrations (50-100%). We also examined the effects of glutathione S-transferase P1 (GSTP1), one of the xenobiotic metabolizing and antioxidant enzymes in the lung, against the cytotoxicity of CSE. Our results indicated that the level of HFL1 cell death was decreased by transfection with a GSTP1 expression vector and was increased by GSTP1 antisense vector transfection. Therefore, transient overexpression and underexpression of GSTP1 appeared to inhibit and enhance the cytotoxic effects of CSE on HFL1 cells, suggesting that GSTP1 may have protective effects against cigarette smoke in the airway cells.