Increased water use efficiency and water productivity of arabidopsis by abscisic acid receptors from Populus canescens.
Increased water use efficiency and water productivity of arabidopsis by abscisic acid receptors from Populus canescens.
复制标题
DOI：
10.1093/aob/mcy225
复制
发表时间：
2019-01
期刊：
影响因子：
4.2
通讯作者：
Michael Papacek;A. Christmann;E. Grill
中科院分区：
文献类型：
--
作者：
Michael Papacek;A. Christmann;E. Grill
背景与目的水分亏缺是限制田间植物生产力的最重要因素。杨树是一种用于第二代生物能源生产的作物，可以在边缘土地上种植，而不会竞争粮食生产的土地使用。杨树对水分的需求量很大，提高其水分利用效率是一个很有吸引力的目标。最近，我们发现增强拟南芥植物激素脱落酸（ABA）特异性受体的表达可以提高拟南芥的水分利用效率和水分生产力，即随着时间的推移，单位水分形成的生物量更多。在本研究中，我们研究了来自杨树的ABA受体是否能提高拟南芥的水分利用效率和水分生产力。方法将杨树ABA受体稳定导入拟南芥，分析其对水分利用效率的影响。生理分析包括生长评估和气体交换测量。本研究的数据与拟南芥中杨树ABA受体的功能一致，该功能导致ABA敏感的种子萌发和根生长。此外，与野生型相比，表达杨树RCAR10而不表达RCAR9的拟南芥株系的水分利用效率提高了26%，而且在生长上几乎没有权衡，这也导致了干旱期间更高的水分生产力。水分利用效率的提高主要是由于气孔导度的降低、叶片边缘更陡的CO2梯度和持续的光合作用导致内在水分利用效率（iWUE）的增加。结论本研究支持了利用杨树ABA受体提高水分利用效率的可行性，并证明了利用异源表达策略提高植物水分生产力的可行性。
Background and Aims Water deficit is the single most important factor limiting plant productivity in the field. Poplar is a crop used for second-generation bioenergy production that can be cultivated on marginal land without competing for land use in food production. Poplar has a high demand for water, which makes improving its water use efficiency (WUE) an attractive goal. Recently, we showed that enhanced expression of specific receptors of arabidopsis for the phytohormone abscisic acid (ABA) can improve WUE in arabidopsis and water productivity, i.e. more biomass is formed per unit of water over time. In this study, we examined whether ABA receptors from poplar can enhance WUE and water productivity in arabidopsis. Methods ABA receptors from poplar were stably introduced into arabidopsis for analysis of their effect on water use efficiency. Physiological analysis included growth assessment and gas exchange measurements. Key Results The data presented here are in agreement with the functionality of poplar ABA receptors in arabidopsis, which led to ABA-hypersensitive seed germination and root growth. In addition, arabidopsis lines expressing poplar RCAR10, but not RCAR9, showed increased WUE by up to 26 % compared with the wild type with few trade-offs in growth that also resulted in higher water productivity during drought. The improved WUE was mediated by reduced stomatal conductance, a steeper CO2 gradient at the leaf boundary and sustained photosynthesis resulting in an increased intrinsic WUE (iWUE). Conclusions The analysis is a case study supporting the use of poplar ABA receptors for improving WUE and showing the feasibility of using a heterologous expression strategy for generating plants with improved water productivity.