Occurrence and temporal variation of antibiotic resistance genes (ARGs) in shrimp aquaculture: ARGs dissemination from farming source to reared organisms
Occurrence and temporal variation of antibiotic resistance genes (ARGs) in shrimp aquaculture: ARGs dissemination from farming source to reared organisms
复制标题
抗生素抗性基因（ARG）的出现和时间变化
DOI：
10.1016/j.scitotenv.2017.07.040
复制
发表时间：
2017-12-31
影响因子：
9.8
通讯作者：
Cao, Yucheng
中科院分区：
文献类型：
--
作者：
Su, Haochang;Liu, Shan;Cao, Yucheng
水环境中抗生素耐药基因(ARGs)的发生和丰富已引起人们的广泛关注。然而，ARGs在水产养殖环境和养殖生物中的时间变化和传播还需要进一步研究。本文调查了中国南部珠江三角洲地区养殖期水源、虾池水、底泥和对虾中ARGs和细菌群落的丰度和多样性。结果表明，在水产养殖样品中，硫酸盐、qnrD、cmlA和Flor是主要的ARGs。养殖期池塘水样中ARGs的丰度呈下降趋势，而沉积物和对虾中的ARGs丰度呈上升趋势。水源水中ARGs总浓度显著高于虾池水(p<；0.05)。池塘水体中ARGs的总浓度与沉积物中ARGs的总浓度呈显著负相关(p<；0.01)。成虾肠道中ARGs的总含量是幼虾的4.48~19.0倍。与水源和池塘水相似，CmlA和Sul1是对虾肠道中的主要ARGs。对虾肠道细菌群落从幼虾到成虾变化较大。本研究的结果表明，在养殖期内，水产养殖中ARGs的丰度有时间上的变化。水源是ARGs向水产养殖环境和养殖生物传播的重要媒介。Sul1可作为珠江三角洲中国南部水产养殖水体和底泥中ARGs的潜在指示剂。这项研究是对水产养殖中ARGs丰度和扩散的时间变化的个案研究，为食品安全和人类健康的潜在风险提供了参考。(C)2017爱思唯尔B.V.保留所有权利。
Considerable attention has been paid to the occurrence and abundance of antibiotic resistance genes (ARGs) in aquatic environments. However, the temporal variation and dissemination of ARGs in aquaculture environments and reared organisms need further study. This study investigated the abundance and diversity of ARGs and bacterial community in water source, shrimp pond water, sediment, and shrimps during the rearing period in Pearl River Delta region, South China. The results showed that sul1, qnrD, cmlA, and floR were the predominant ARGs in the aquaculture samples. A trend of decreasing abundance of ARGs was observed for pond water samples during the rearing period, whereas an increasing trend was observed in the sediment and shrimp samples. The total concentration of ARGs in water source was significantly higher than that in shrimp pond water (p < 0.05). A significant negative correlation was found between the total concentrations of ARGs in pond waters and sediments (p < 0.01). The total abundances of ARGs in intestinal tract of adult shrimps were 4.48-19.0 times higher than those in juvenile shrimps. Similar to water source and pond water, cmlA and sul1 were the predominant ARGs in shrimp intestinal tract. The bacterial community in the shrimp intestinal tract changed greatly from juvenile to adult. The results of the present study indicated that the abundances of ARGs in aquaculture varied temporally during the rearing period. Water source was an important medium disseminating ARGs to the aquaculture environments and reared organisms. Sul1 could be used as a potential indicator for ARGs in both water and sediment in aquaculture in the estuary of the Pearl River Delta, South China. This study represents a case study for the temporal variation of abundance and dissemination of ARGs in aquaculture and is a reference for potential risks to food safety and human health. (C) 2017 Elsevier B.V. All rights reserved.