Haemolysis of Red Blood Cells In Vitro and In Vivo Induced by Ultrasound at 0.75 MHz and at Therapeutic Intensity Levels
Haemolysis of Red Blood Cells In Vitro and In Vivo Induced by Ultrasound at 0.75 MHz and at Therapeutic Intensity Levels
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0.75 MHz 超声和治疗强度水平引起的体外和体内红细胞溶血
DOI：
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发表时间：
1983
期刊：
影响因子：
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通讯作者：
D. Watmough
中科院分区：
文献类型：
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作者：
Y. S. Wong;D. Watmough
Nyborg(1)对超声修饰或损伤生物材料的物理机制进行了全面的讨论。这些包括微流、热效应和一系列空化现象。众所周知，声激气泡可引起红细胞溶血(2)。微流对红细胞造成的剪切应力足以破坏细胞膜。当处理后的样品随后用分光光度法测量时，血红蛋白的释放引起光学吸光度的变化。Nyborg(1)和其他人已经注意到，在不透明的大块动物组织中，关于空化的信息很少。本文描述的工作目的是将未经修改的治疗发生器（Sonacel, Rank Stanley, Cox, Ware，赫特福德郡，英格兰）的超声引导到小动物的心脏，试图检测或排除体内溶血。这项研究的需要源于现在广泛使用超声作为治疗各种疾病的治疗剂(3)。此外，利用聚焦或重叠的超声场治疗恶性肿瘤的可能性也引起了相当大的兴趣(4)，以期产生局部热疗。显然，了解这种治疗是否可能产生任何不利的副作用是至关重要的。
The physical mechanisms by which ultrasound can modify or damage biological materials has been comprehensively discussed by Nyborg(1). These include microstreaming, thermal effects and a range of cavitation type phenomena. It is well known that an acoustically excited bubble can cause haemolysis of red cells(2). The shearing stresses on the red cells caused by associated microstreaming is sufficient to damage cell membranes. Release of haemoglobin causes changes in optical absorbance when the treated samples are subsequently measured by spectrophotometry. Nyborg(1) and others have remarked that there is little information on cavitation in bulk animal tissues which are characteristically opaque. The objective of the work described here was to direct ultrasound from an unmodified therapeutic generator (Sonacel, Rank Stanley, Cox, Ware, Hertfordshire, England) at the hearts of small animals in an attempt to detect or exclude haemolysis in vivo. The need for this investigation arises from the now widespread use of ultrasound as a therapeutic agent to treat a variety of conditions(3). Furthermore, there is considerable interest in the possibility of treating malignant tumors with focussed or overlapping ultrasound fields(4) with a view to producing local hyperthermia. Clearly it is vital to know if there are likely to be any untoward side-effects arising from such treatment.