PEPTIDE CHAIN TERMINATION, CODON, PROTEIN FACTOR, AND RIBOSOMAL REQUIREMENTS
PEPTIDE CHAIN TERMINATION, CODON, PROTEIN FACTOR, AND RIBOSOMAL REQUIREMENTS
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DOI：
10.1101/sqb.1969.034.01.054
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发表时间：
1969-01-01
期刊：
影响因子：
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通讯作者：
MILMAN, G
中科院分区：
文献类型：
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作者：
CASKEY, T;SCOLNICK, E;MILMAN, G
生化研究的结果（Last等人，一九六七年; Capecchi，1967年; Bretscher，1968）和遗传学研究（Weigert和Garen，1965; Brenner，斯特雷顿和Kaplan，1965）表明，肽链的合成在信使RNA密码子UAA或UAG翻译后终止。遗传证据还表明UGA作为屏障或终止密码子起作用（Brenner，巴内特，Katz和Crick，1967; Weigert，Lanka和Garen，1967）。通过用随机排序或测序的多核糖核苷酸刺激无细胞蛋白质合成，研究了肽链终止的机制（Takanami和YH Yan，1965; Bretscher等人，1965; Ganoza和Nakamoto，1966; Morgan，威尔斯和Khorana，1966; KSssel，1968）或病毒RNA（Capecchi，1967; Bretscher，1968）。UAA或UAG的翻译导致游离肽从核糖体释放。Capecchi（1967）部分纯化了琥珀密码子UAG翻译后从核糖体释放新生肽链所需的因子。
Results of biochemical studies (Last et al., 1967; Capecchi, 1967; Bretscher, 1968) and genetic studies (Weigert and Garen, 1965; Brenner, Stretton, and Kaplan, 1965) show that the synthesis of a peptide chain is terminated upon translation of messenger RNA codons UAA or UAG. Genetic evidence also indicates UGA functions as a barrier or terminator codon (Brenner, Barnett, Katz, and Crick, 1967; Weigert, Lanka, and Garen, 1967).The mechanism of peptide chain termination has been investigated by stimulating cell-free protein synthesis with randomly ordered or sequenced polyribonucleotides (Takanami and YH Yan, 1965; Bretscher et al., 1965; Ganoza and Nakamoto, 1966; Morgan, Wells, and Khorana, 1966; KSssel, 1968), or viral RNA (Capecchi, 1967; Bretscher, 1968). Translation of UAA or UAG results in the release of free peptides from ribosomes. Capecchi (1967) partially purified a factor required for the release of nascent peptide chains from ribosomes upon the translation of the amber codon, UAG.