Sequential Onset of Presynaptic Molecules during Olfactory Sensory Neuron Maturation
Sequential Onset of Presynaptic Molecules during Olfactory Sensory Neuron Maturation
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DOI：
10.1002/cne.22094
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发表时间：
2009-09-20
影响因子：
2.5
通讯作者：
Firestein, Stuart
中科院分区：
文献类型：
--
作者：
Marcucci, Florencia;Zou, Dong-Jing;Firestein, Stuart
分化的嗅觉感觉神经元表达特定的突触前蛋白，包括参与神经递质运输的酶和参与突触囊泡的运输和释放的蛋白。研究这些突触前蛋白的调控将有助于阐明最终导致突触形成的突触前分化过程。据推测，在嗅球中感觉神经元的轴突和二级神经元的树突之间形成突触是感觉神经元成熟过程中的关键步骤。研究突触发生和感觉神经元成熟之间关系的一种方法是通过嗅觉神经元谱系检查突触分子的表达模式。为此，我们设计了特异性原位杂交探针，靶向参与突触前囊泡释放的蛋白质信使。我们的研究结果表明，随着它们的成熟，小鼠嗅觉神经元顺序表达特定的突触前基因。此外，这些突触前基因的表达的不同模式表明突触前发育中存在离散的步骤：编码支架蛋白的基因显示早期表达，而编码囊泡释放调控蛋白的基因表达开始较晚。特别是，谷氨酸能神经元囊泡谷氨酸转运体2的标志分子显示了最新的表达。此外，与嗅球中的目标接触并不控制突触前蛋白基因的表达，这表明嗅觉感觉神经元遵循内在的发育程序。J. Comp.神经元516：187-198，2009. (C)2009 Wiley-Liss，Inc.
Differentiated olfactory sensory neurons express specific presynaptic proteins, including enzymes involved in neurotransmitter transport and proteins involved in the trafficking and release of synaptic vesicles. Studying the regulation of these presynaptic proteins will help to elucidate the presynaptic differentiation process that ultimately leads to synapse formation. It has been postulated that the formation of a synapse between the axons of the sensory neurons and the dendrites of second order neurons in the olfactory bulb is a critical step in the processes of sensory neuron maturation. One approach to study the relationship between synaptogenesis and sensory neuron maturation is to examine the expression patterns of synaptic molecules through the olfactory neuron lineage. To this end we designed specific in situ hybridization probes to target messengers for proteins involved in presynaptic vesicle release. Our findings show that, as they mature, mouse olfactory neurons sequentially express specific presynaptic genes. Furthermore, the different patterns of expression of these presynaptic genes suggest the existence of discrete steps in presynaptic development: genes encoding proteins involved in scaffolding show an early onset of expression, whereas expression of genes encoding proteins involved in the regulation of vesicle release starts later. In particular, the signature molecule for glutamatergic neurons vesicle glutamate transporter 2 shows the latest onset of expression. In addition, contact with the targets in the olfactory bulb is not controlling presynaptic protein gene expression, suggesting that olfactory sensory neurons follow an intrinsic program of development. J. Comp. Neurol. 516:187-198, 2009. (C) 2009 Wiley-Liss, Inc.