Investigations of free and elastin-bound fluorescent substances present in the atherosclerotic lipid and calcium-plaques.
Investigations of free and elastin-bound fluorescent substances present in the atherosclerotic lipid and calcium-plaques.
复制标题
研究动脉粥样硬化脂质和钙斑中存在的游离和弹性蛋白结合的荧光物质。
作者：
I. Banga;M. Bihari
对动脉粥样硬化血管内膜斑块中两种荧光物质的性质和行为进行了研究。第一个峰的最大激活和发射波长为350/405，这是纯弹性蛋白的特征，而另一个峰的比荧光为380/450。后者只有一小部分可以很容易地从斑块中提取出来，而大部分被紧密地结合在一个复合体中。经弹性酶消化后，荧光物质和相关疏水性多肽可以用氯仿：甲醇3：1提取，弹性酶处理使弹性蛋白和相关蛋白都被水解。这种荧光化合物在发射方面与其他组织色素相似，在硫代巴比妥酸测试中呈阳性反应，这是丙二醛的红色斑点。
The nature and behavior of two fluorescent substances, present in the intimal plaques of atherosclerotic human aortas were studied. The maximum activation and emission wavelength of the first peak was 350/405 which is characteristic for pure elastin, while the specific fluorescence of the other peak was 380/450. Only a small part of the latter was readily extractable from the plaques while the major portion was tightly bound in a complex. After digestion with elastase the fluorescent material and associated hydrophobic peptides could be extracted by chloroform:methanol 3:1 The elastase treatment hydrolyzed both elastin and associated proteins. The fluorescent compound resembled other tissue pigments in its emission, and in giving a positive reaction in the thiobarbituric acid test, a red spot characteristic for malonaldehyde.