Infection of primary human bronchial epithelial cells by Haemophilus influenzae:: Macropinocytosis as a mechanism of airway epithelial cell entry
Infection of primary human bronchial epithelial cells by Haemophilus influenzae:: Macropinocytosis as a mechanism of airway epithelial cell entry
复制标题
DOI：
10.1128/iai.67.8.4161-4170.1999
复制
发表时间：
1999-08-01
影响因子：
3.1
通讯作者：
Apicella, MA
中科院分区：
文献类型：
--
作者：
Ketterer, MR;Shao, JQ;Apicella, MA
不可分型流感嗜血杆菌是一种感染呼吸道上皮的人类特有病原体。我们已经开始了研究，以探索不可分型的H的相互作用。通过电子和共聚焦显微镜观察流感病毒株2019与原代人气道上皮细胞的关系。原代人气道细胞培养物在玻璃胶原包被的盖玻片上或在空气-流体界面处的半透膜上建立为单层。扫描电子显微镜表明细菌粘附于群体中的无纤毛细胞。感染细胞的表面显示出细胞骨架重排的证据，表现为微绒毛和板状伪足向细菌延伸并与细菌接合。共聚焦显微镜分析表明，感染诱导肌动蛋白聚合增加皮质肌动蛋白以及周围的细菌肌动蛋白链的证据。透射电子显微镜分析显示，板状伪足和微绒毛周围的生物体，以及生物体粘附在细胞表面。这些研究还证明了气道细胞内空泡内存在细菌。用德克萨斯红标记的葡聚糖（分子量，70,000）进行的共聚焦显微镜研究表明，H。流感细胞通过巨胞饮过程进入细胞。这些研究表明，不可分型的H。流感病毒可引发人气道上皮细胞内的细胞骨架重排，导致细菌通过巨胞饮作用内化到无纤毛的人气道上皮细胞内。
Nontypeable Haemophilus influenzae is an exclusive human pathogen which infects the respiratory epithelium. We have initiated studies to explore the interaction of the nontypeable H. influenzae strain 2019 with primary human airway epithelial cells by electron and confocal microscopy, Primary human airway cell cultures were established as monolayers on glass collagen-coated coverslips or on semipermeable membranes at an air-fluid interface, Scanning electron microscopy indicated that bacteria adhered to nonciliated cells in the population. The surface of infected cells showed evidence of cytoskeletal rearrangements manifested by microvilli and lamellipodia extending toward and engaging bacteria. Confocal microscopic analysis demonstrated that infection induced actin polymerization with an increase in cortical actin as well as evidence of actin strands around the bacteria. Transmission electron microscopic analysis showed lamellipodia and microvilli surrounding organisms, as well as organisms adherent to the cell surface. These studies also demonstrated the presence of bacteria within vacuoles inside of airway cells. Confocal microscopic studies with Texas red-labeled dextran (molecular weight, 70,000) indicated that H. influenzae cells were entering cells by the process of macropinocytosis. These studies indicate that nontypeable H. influenzae can initiate cytoskeletal rearrangement within human airway epithelium, resulting in internalization of the bacteria within nonciliated human airway epithelial cells by the process of macropinocytosis.