Genetic diversity in lac resin-secreting insects belonging to Kerria spp., as revealed through ISSR markers
Genetic diversity in lac resin-secreting insects belonging to Kerria spp., as revealed through ISSR markers
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DOI：
10.1016/j.bse.2011.01.016
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发表时间：
2011-04-01
影响因子：
1.6
通讯作者：
Ramani, Ranganathan
中科院分区：
文献类型：
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作者：
Saha, Dipnarayan;Ranjan, Sanjeev Kumar;Ramani, Ranganathan
印度紫胶虫Kerria lacca在印度被用于商业化紫胶生产，紫胶具有多种工业应用。由于当地气候模式的差异越来越大，该物种的许多地理种族正面临灭绝的威胁。因此，有必要记录和保护紫胶虫的生物多样性，特别是适应当地的气候条件和宿主物种。本文对20个重要经济种的遗传多样性进行了研究。采用简单重复序列（Inter-Simple Sequence Repeat，ISSR）技术进行分析。17个ISSR引物在紫胶虫品系中产生了96.1%的遗传多态性。聚类分析将20个品系分为4大类，相似系数在0.25~0.81之间。前三个主坐标揭示了总遗传变异的43.1%。以上结果表明，紫胶虫品系间存在显著的遗传变异，可用于紫胶虫的遗传改良。(C)2011爱思唯尔有限公司保留所有权利。
The Indian lac insect Kerria lacca is harnessed in India for commercial production of lac, which has diversified industrial applications. Many of the geographical races of this species are under threat of extinction due to increasingly drastic local deviations in climate patterns. Thus, there is need for documentation and conservation of the lac insect biodiversity adapted especially for local climatic conditions and host species. The genetic diversity among twenty lines of commercially important Kerria spp. was analyzed using Inter-Simple Sequence Repeat (ISSR) technique. Seventeen ISSR primers produced 96.1% genetic polymorphism in the lac insect lines under study. The clustering dendrogram segregated the twenty lines into four major clusters with similarity coefficients between 0.25 and 0.81. The first three principal coordinates revealed 43.1% of the total genetic variation. The above results reveal significant genetic variability in the lines, which could be used for genetic improvement of lac insects. (C) 2011 Elsevier Ltd. All rights reserved.