Transcriptome profiling and differential expression analysis of the immune-related genes during the acute phase of infection with Mycobacterium marinum in the goldfish (Carassius auratus L.)
Transcriptome profiling and differential expression analysis of the immune-related genes during the acute phase of infection with Mycobacterium marinum in the goldfish (Carassius auratus L.)
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金鱼感染海洋分枝杆菌急性期免疫相关基因的转录组分析和差异表达研究
DOI：
10.1016/j.aquaculture.2020.736198
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发表时间：
2021-02-25
期刊：
影响因子：
4.5
通讯作者：
Wang，Yajun
中科院分区：
文献类型：
--
作者：
Li，Chao;Jiang，Jianhu;Wang，Yajun
金鱼（Carassius auratusL.）已被用作研究先天免疫系统和宿主-病原体相互作用的模式生物。海分枝杆菌是一种革兰氏阳性细菌，已被证明可以感染许多鱼类。增加对宿主防御支原体的分子机制的了解。 marinum，我们在感染后 24 小时通过 Illumina 测序对金鱼的脾脏进行了转录组分析。新组装鉴定出 83,674 个 unigene，其中 69,050 个 (82.52.9%) 在公共数据库（GO、KEGG、COG、NR、SwissProt 和 Pfam）中成功注释。 M 之间基因表达谱的比较。经海藻刺激的鱼和注射 PBS 的鱼（假对照）显示出 4200 个差异表达基因 (DEG)，其中 2211 个上调，1989 个下调。 DEGs富集于多种免疫相关通路，如Toll样受体信号通路、NOD样受体信号通路、NF-κB信号通路、Jak-STAT信号通路、细胞因子-细胞因子受体相互作用通路等。本研究中获得的数据为金鱼受到支原体攻击的急性免疫反应提供了有价值的信息。 marinum，这将有助于进一步调查针对M的策略。硬骨鱼中的海洋感染。
Goldfish (Carassius auratusL.) have been used as a model organism to study innate immune system and host-pathogen interactions.Mycobacterium marinumis a Gram-positive bacterium that has been shown to infect many fish species. To increase knowledge of the molecular mechanisms of the host defense againstM. marinum, we conducted transcriptome analysis of spleen in goldfish 24 h post-infectionviaIllumina sequencing. Thede novoassembly resulted in the identification of 83,674 unigenes, with 69,050 (82.52.9%) successfully annotated in public databases (GO, KEGG, COG, NR, SwissProt and Pfam). Comparison of gene expression profiles betweenM. marinum-stimulated fish and PBS-injected fish (sham control) revealed 4200 differentially expressed genes (DEGs), with 2211 up-regulated and 1989 down-regulated. The DEGs were enriched in multiple immune-related pathways such as Toll-like receptor signaling pathway, NOD-like receptor signaling pathway, NF-κB signaling pathway, Jak-STAT signaling pathway, and cytokine-cytokine receptor interaction pathway. The data obtained in the present study offers valuable information for acute immune response of goldfish challenged withM. marinum, which will facilitate further investigations on strategies againstM. marinuminfection in teleosts.