Novel glucose sensor based on enzymeimmobilized 81° tilted fiber grating
Novel glucose sensor based on enzymeimmobilized 81° tilted fiber grating
复制标题
基于酶固定化81°倾斜光纤光栅的新型葡萄糖传感器
DOI：
10.1364/oe.22.030571
复制
发表时间：
2014-12-15
期刊：
影响因子：
3.8
通讯作者：
Zhang, Lin
中科院分区：
文献类型：
--
作者：
Luo, Binbin;Yan, Zhijun;Zhang, Lin
我们展示了一种新型葡萄糖传感器，其基于光纤光栅，具有过度倾斜的折射率条纹结构，其表面经过葡萄糖氧化酶（GOD）修饰。氨丙基三乙氧基硅烷 (APTES) 用作后续 GOD 固定的结合位点。使用共焦显微镜和荧光显微镜来评估纤维表面改性的有效性。 APTES和GOD与纤维表面结合后以及葡萄糖检测过程中传感器的共振波长均出现红移。共振波长的红移对葡萄糖浓度表现出良好的线性响应，在0.0至3.0mg/ml的极低浓度范围内灵敏度为0.298nm.(mg/ml)(-1)。与之前报道的基于 GOD 固定长周期光栅 (LPG) 的葡萄糖传感器相比，基于 81 度倾斜光纤光栅 (81 度-TFG) 的传感器在传感响应中表现出更低的热串扰效应、更好的线性度和更高的 Q 因子。此外，通过增加光栅长度和/或选择高阶包层模式进行检测，可以进一步提高其对葡萄糖浓度的灵敏度。所提出的基于 81 度-TFG 的技术可以开发为灵敏、无标签和微结构传感器，用于食品安全、疾病诊断、临床分析和环境监测等领域。 (C) 2014年美国光学学会
We demonstrate a novel glucose sensor based on an optical fiber grating with an excessively tilted index fringe structure and its surface modified by glucose oxidase (GOD). The aminopropyltriethoxysilane (APTES) was utilized as binding site for the subsequent GOD immobilization. Confocal microscopy and fluorescence microscope were used to provide the assessment of the effectiveness in modifying the fiber surface. The resonance wavelength of the sensor exhibited red-shift after the binding of the APTES and GOD to the fiber surface and also in the glucose detection process. The red-shift of the resonance wavelength showed a good linear response to the glucose concentration with a sensitivity of 0.298nm.(mg/ml)(-1) in the very low concentration range of 0.0 similar to 3.0mg/ml. Compared to the previously reported glucose sensor based on the GOD-immobilized long period grating (LPG), the 81 degrees tilted fiber grating (81 degrees-TFG) based sensor has shown a lower thermal cross-talk effect, better linearity and higher Q-factor in sensing response. In addition, its sensitivity for glucose concentration can be further improved by increasing the grating length and/or choosing a higher-order cladding mode for detection. Potentially, the proposed techniques based on 81 degrees-TFG can be developed as sensitive, label free and micro-structural sensors for applications in food safety, disease diagnosis, clinical analysis and environmental monitoring. (C) 2014 Optical Society of America