EVIDENCE FOR THE MAILLARD REACTION IN RAT LUNG COLLAGEN AND ITS RELATIONSHIP WITH SOLUBILITY AND AGE
EVIDENCE FOR THE MAILLARD REACTION IN RAT LUNG COLLAGEN AND ITS RELATIONSHIP WITH SOLUBILITY AND AGE
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DOI：
10.1016/0925-4439(95)00069-g
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发表时间：
1995-08-15
影响因子：
6.2
通讯作者：
PRAT, J
中科院分区：
文献类型：
--
作者：
BELLMUNT, MJ;PORTERO, M;PRAT, J
本研究探讨了年龄相关的胶原蛋白溶解度和胶原蛋白相关荧光的变化，以及它们与美拉德反应的关系。作为大鼠肺样品的胶原蛋白纯化的结果，我们获得了具有不同溶解度的两个胶原蛋白池。胶原在两种组分间的相对分布呈时间依赖性，较小和较难溶解的组分所占比例随时间增加而增加（r = 0.73，P < 0.0001）。随着年龄的增长，其Exc 335 nm/Em 385 nm处的荧光强度和戊糖甙总量均增加（r = 0.66，P < 0.002; r = 0.69，P < 0.01）。每毫克胶原蛋白的荧光和戊糖苷的平均值分别是可溶性较低部分的6倍和10倍。随着年龄的增长，每毫克胶原中的戊糖苷含量增加（r = 0.59，P < 0.03）。我们的研究结果表明，大鼠肺胶原中存在戊糖苷。此外，它的积累在不太可溶的馏分建议美拉德反应产物之间的关系，胶原蛋白溶解度的物理化学变化，和老化过程中的大鼠肺。
This study investigated age-related changes in collagen solubility and collagen-linked fluorescence, and their relationship with the Maillard reaction. As a result of the collagen purification of rat lung samples, we obtained two pools of collagen with different degrees of solubility. The relative distribution of collagen between these two fractions was time-dependent, and the proportion of the smaller and less soluble fraction increased with time (r = 0.73, P < 0.0001). In this fraction, the intensity of fluorescence at Exc 335 nm/Em 385 nm, and the total amount of pentosidine increased with age (r = 0.66, P < 0.002, and r = 0.69, P < 0.01, respectively). The mean values for fluorescence and pentosidine per milligram of collagen were, respectively, six and ten times greater in the less soluble fraction. In this fraction the pentosidine per milligram of collagen increased with age (r = 0.59, P < 0.03). Our results demonstrated the presence of pentosidine in rat lung collagen. Moreover, its accumulation in the less soluble fraction suggested a relationship between Maillard reaction products, physico-chemical changes in collagen solubility, and the ageing process in rat lungs.