Effect of collagen I and fibronectin on the adhesion, elasticity and cytoskeletal organization of prostate cancer cells
Effect of collagen I and fibronectin on the adhesion, elasticity and cytoskeletal organization of prostate cancer cells
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DOI：
10.1016/j.bbrc.2010.10.034
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发表时间：
2010-11-12
影响因子：
3.1
通讯作者：
Clausen-Schaumann, Hauke
中科院分区：
文献类型：
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作者：
Docheva, Denitsa;Padula, Daniela;Clausen-Schaumann, Hauke
尽管进行了大量的研究工作，但前列腺癌骨转移的确切机制仍不完全清楚。一些研究表明，骨细胞产生的特定基质，如胶原蛋白I，支持癌细胞侵袭。为探讨Ⅰ型胶原（COL1）和纤连蛋白（FN）对前列腺癌细胞粘附、细胞弹性和细胞骨架结构的影响，将骨髓来源的前列腺癌细胞系（PC3）和淋巴结来源的前列腺癌细胞系（LNCaP）分别培养于COL1和FN（对照蛋白）上。通过使用定量粘附测定和时间推移分析，发现PC 3而不是LNCaP在COL1上粘附强烈并且更多地扩散。接着，通过原子力显微镜（AFM）评估PC 3和LNCaP，并计算平坦形状因子和细胞杨氏模量。形状分析显示，与LNCaP相比，当在COL1上生长时，PC3显著更平坦。在一般情况下，PC 3也显着比LNCaP刚度，而且，它们的刚度增加后，与COL1的相互作用。由于细胞硬度强烈依赖于肌动蛋白组织，基于鬼笔环肽的肌动蛋白染色进行，并显示，两种细胞类型以及两种不同的基质蛋白，只有PC 3上生长的COL1形成强大的肌动蛋白cytokerelastics.In结论，我们的研究表明，PC 3细胞对COL1有很强的亲和力。在这种基质蛋白上，细胞牢固地粘附并经历特定的细胞扁平化。此外，随着PC3与COL1接触的建立，由于深刻的细胞骨架重排，观察到PC3刚度显著增加。(C)2010年爱思唯尔公司All rights reserved.
Despite of intensive research efforts, the precise mechanism of prostate cancer metastasis in bone is still not fully understood. Several studies have suggested that specific matrix production by the bone cells, such as collagen I, supports cancer cell invasion. The aim of this study was to investigate the effect of collagen I (COL1) and fibronectin (FN) on cell adhesion, cell elasticity and cytoskeletal organization of prostate cancer cells.Two cell lines, bone marrow- (PC3) and lymph node-derived (LNCaP) were cultivated on COL1 and FN (control protein). By using a quantitative adhesion assay and time-lapse analysis, it was found that PC3, but not LNCaP, adhered strongly and were more spread on COL1. Next, PC3 and LNCaP were evaluated by atomic force microscopy (AFM) and flatness shape factor and cellular Young's modulus were calculated. The shape analysis revealed that PC3 were significantly flatter when grown on COL1 in comparison to LNCaP. In general, PC3 were also significantly stiffer than LNCaP and furthermore, their stiffness increased upon interaction with COL1. Since cell stiffness is strongly dependent on actin organization, phalloidin-based actin staining was performed and revealed that, of the two cell types as well as the two different matrix proteins, only PC3 grown on COL1 formed robust actin cytoskeleton.In conclusion, our study showed that PC3 cells have a strong affinity towards COL1. On this matrix protein, the cells adhered strongly and underwent a specific cell flattening. Moreover, with the establishment of PC3 contact to COL1 a significant increase of PC3 stiffness was observed due to a profound cytoskeletal rearrangement. (C) 2010 Elsevier Inc. All rights reserved.