CAPILLARY ZONE ELECTROPHORETIC SEPARATIONS OF PROTEINS IN POLYETHYLENE GLYCOL-MODIFIED CAPILLARIES
CAPILLARY ZONE ELECTROPHORETIC SEPARATIONS OF PROTEINS IN POLYETHYLENE GLYCOL-MODIFIED CAPILLARIES
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DOI：
10.1016/s0021-9673(00)94190-6
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发表时间：
1989-06-02
期刊：
影响因子：
--
通讯作者：
POPPE, H
中科院分区：
文献类型：
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作者：
BRUIN, GJM;CHANG, JP;POPPE, H
熔融二氧化硅毛细管的壁用γ-缩水甘油氧基丙基三甲氧基硅烷和聚乙二醇600，以减少蛋白质的毛细管区带电泳分离中的壁吸附的影响。它示出了一个显着的减少，在吸附获得和电渗流也减少。对于所研究的蛋白质，在pH 3-5范围内获得对称峰。然而，由于塔板数低于理论预期值，因此仍会发生一些吸附。在较高的pH值下，观察到明显的峰变形和分辨率急剧下降。该程序允许快速有效地分离蛋白质混合物，适合在指定的pH范围内分离，并且涂层显示出良好的稳定性。
Fused-silica capillaries were wall modified with .gamma.-glycidoxypropyltrimethoxysilane and polyethylene glycol 600 in order to decrease the influence of wall adsorption in capillary zone electrophoretic separations of proteins. It is shown that a significant decrease in adsorption is obtained and electro-osmotic flow is also diminished. For the proteins studied, symmetrical peaks were obtained in the pH range 3-5. However, some adsorption still occurs as the plate numbers are below theoretical expectations. At higher pH values appreciable peak deformations and drastic decreases in resolving power are observed. The procedure allows the rapid and efficient separation of protein mixtures suitable for separation in the indicated pH range, and the coating shows a good stability.