Capsicum annuum WRKY protein CaWRKY1 is a negative regulator of pathogen defense
Capsicum annuum WRKY protein CaWRKY1 is a negative regulator of pathogen defense
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DOI：
10.1111/j.1469-8137.2007.02310.x
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发表时间：
2008-01-01
期刊：
影响因子：
9.4
通讯作者：
Choi, Doil
中科院分区：
文献类型：
--
作者：
Oh, Sang-Keun;Bek, Kwang-Hyun;Choi, Doil
植物对病原菌的反应是通过调节一系列信号通路来微调防御相关基因的转录激活。本研究的目的是确定辣椒WRKY锌指域转录因子1(CaWRKY1)在防御中的作用。以往的研究发现，CaWRKY1基因在辣椒(C.annuum)叶片中被不亲和和亲和的病原菌快速诱导，但由于WRKY家族网络的复杂性，CaWRKY1在辣椒叶片中的防御功能尚不清楚。过量表达CaWRKY1的转基因植株对烟草花叶病毒和假单胞菌注射器PV的侵染表现出加速的细胞死亡。塔巴奇。在水杨酸(SA)处理后过表达CaWRKY1的转基因植株中观察到较低水平的致病相关基因诱导。这项研究表明，新鉴定的CaWRKY1是由病原菌侵染和信号分子SA强烈诱导的，在病原菌攻击减弱时作为调节因子关闭系统获得性抗性，并防止在次适浓度SA下假激活防御反应。
Plants respond to pathogens by regulating a network of signaling pathways that fine-tune transcriptional activation of defense-related genes.The aim of this study was to determine the role of Capsicum annuum WRKY zinc finger-domain transcription factor 1 (CaWRKY1) in defense. In previous studies, CaWRKY1 was found to be rapidly induced in C. annuum (chili pepper) leaves by incompatible and compatible pathogen inoculations, but the complexity of the network of the WRKY family prevented the function of CaWRKY1 in defense from being elucidated.Virus-induced gene silencing of CaWRKY1 in chili pepper leaves resulted in decreased growth of Xanthomonas axonopodis pv. vesicatoria race 1. CaWRKY1-overexpressing transgenic plants showed accelerated hypersensitive cell death in response to infection with tobacco mosaic virus and Pseudomonas syringe pv. tabaci. Lower levels of pathogenesis-related gene induction were observed in CaWRKY1-overexpressing transgenic plants following salicylic acid (SA) treatments.This work suggests that the newly characterized CaWRKY1, which is strongly induced by pathogen infections and the signal molecule SA, acts as a regulator to turn off systemic acquired resistance once the pathogen challenge has diminished and to prevent spurious activation of defense responses at suboptimal concentrations of SA.