NHE2 and NHE3 are human and rabbit intestinal brush-border proteins
NHE2 and NHE3 are human and rabbit intestinal brush-border proteins
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DOI：
10.1152/ajpgi.1996.270.1.g29
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发表时间：
1996-01-01
影响因子：
4.5
通讯作者：
Donowitz, M
中科院分区：
文献类型：
--
作者：
Hoogerwerf, WA;Tsao, SC;Donowitz, M
兔NHE 2和NHE 3是两种上皮同种型Na+/H+交换体（NHE），其信息分别主要和完全存在于肾、肠和胃粘膜中。目前的研究使用Western分析和免疫组织化学来鉴定和表征肠上皮细胞中NHE 2和NHE 3的顶膜与基底膜分布。基于Western分析，NHE 2和NHE 3均存在于小肠刷状缘而非基底外侧膜。NHE 2和NHE 3都是85-kDa蛋白质。与Western分析一致，NHE 2和NHE 3免疫定位于人空肠和回肠中绒毛上皮细胞的刷状缘而非基底外侧膜，而非杯状细胞，以及升结肠和降结肠和直肠中的表面上皮细胞。此外，NHE 2和NHE 3以少量存在于人空肠、回肠、升结肠和降结肠以及直肠的隐窝细胞刷状缘中。在兔空肠、回肠和升结肠中，NHE 2和NHE 3存在于上皮细胞的刷状缘中而不是杯状细胞中，再次在绒毛（小肠）/表面细胞（结肠）中比在隐窝中多得多。NHE 2而非NHE 3存在于兔降结肠表面细胞的刷状缘中，并且少量存在于隐窝细胞中。NHE 2和NHE 3都是人和兔小肠和结肠上皮细胞刷状缘Na+/H+交换器同种型，其共定位于除缺乏NHE 3的兔降结肠之外的所有肠段。
Rabbit NHE2 and NHE3 are two epithelial isoform Na+/H+ exchangers (NHE), the messages for which are found predominantly and entirely, respectively, in renal, intestinal, and gastric mucosa. The current studies used Western analysis and immunohistochemistry to identify and characterize the apical vs. basolateral membrane distribution of NHE2 and NHE3 in intestinal epithelial cells. Based on Western analysis, NHE2 and NHE3 both are present in brush-border but not basolateral membranes of small intestine. Both NHE2 and NHE3 are 85-kDa proteins. Consistent with Western analysis, NHE2 and NHE3 are immunolocalized to the brush-border but not basolateral membranes of villus epithelial cells, but not goblet cells, in human jejunum and ileum and in surface epithelial cells in the ascending and descending colon and rectum. In addition, NHE2 and NHE3 are present in small amounts in the crypt cell brush border of human jejunum, ileum, ascending and descending colon, and rectum. In rabbit jejunum, ileum, and ascending colon, NHE2 and NHE3 are present in the brush border of epithelial and not goblet cells, again much more in the villus (small intestine)/surface cells (colon) than the crypt. NHE2 but not NHE3 is present in the brush border of rabbit descending colon surface cells and in small amounts in crypt cells. NHE2 and NHE3 are both human and rabbit small intestinal and colonic epithelial cell brush-border Na+/H+ exchanger isoforms that colocalize in all intestinal segments except rabbit descending colon, which lacks NHE3.