EFFECT OF DENERVATION ON LIMB GROWTH
EFFECT OF DENERVATION ON LIMB GROWTH
复制标题
DOI：
10.1002/jor.1100070218
复制
发表时间：
1989-03-01
影响因子：
2.8
通讯作者：
DIETZ, FR
中科院分区：
文献类型：
--
作者：
DIETZ, FR
本研究以尖音蛙蝌蚪为研究对象，探讨坐骨神经完全或部分切断对胫骨生长和足生长的影响。完全去坐骨神经。在阶段XIV，XVII和XX的pipiens，它们在阶段XVII，XX和XXIV被杀死。部分去神经包括在第XVII和XX阶段切断腓神经或胫神经，并在第XX和XXIV阶段处死。实验动物和对照动物的分析包括（a）定量轴突计数，（B）胫骨长度，（c）胫骨中段横截面积，（d）胫骨中段皮质厚度，（e）胫骨中段软骨原基横截面积，（f）足轮廓面积，和（g）骨细胞数量和骨细胞密度。完全和部分去神经支配均对骨骼和足部生长产生显著影响：（a）骨骼长度减少，（B）骨横截面积减少而无皮质变薄，（c）软骨原基横截面积增加，以及（d）足部尺寸减小。本实验证实了神经对骨生长发育和足部生长的营养作用。这种作用的机制尚不清楚，但数据表明去神经骨骼的成熟速度减慢。有缺陷的周围神经-肢体组织相互作用可能导致人类畸形，如特发性马蹄内翻足和特发性肢体长度不等。
This study used Rana pipiens tadpoles to assess the effect of complete and partial sciatic denervation on tibial bone growth and foot growth. Complete sciatic denervation was performed in R. pipiens at Stages XIV, XVII, and XX and they were killed at Stages XVII, XX, and XXIV. Partial denervation consisted of peroneal or tibial nerve sectioning at Stages XVII and XX with killing at Stages XX and XXIV. Analysis of experimental animals and controls consisted of (a) quantitative axon counts, (b) tibial length, (c) midtibial cross-sectional area, (d) midtibial cortical thickness, (e) midtibial cartilage anlage cross-sectional area, (f) foot silhouette area, and (g) osteocyte number and osteocyte density. Both complete and partial denervation resulted in significant effects on bone and foot growth: (a) decreased bone length, (b) decreased cross-sectional bone area without cortical thinning, (c) increased cartilage anlage cross-sectional area, and (d) decreased foot size. This experiment demonstrated a trophic effect of nerve on bone growth and development and foot growth. The mechanism of this action is unknown but the data suggests a slowed rate of maturation in denervated bones. The possibility exists that defective peripheral nerve-limb tissue interactions may cause human deformities such as idiopathic clubfoot and idiopathic limb length discrepancy.