Inducible expression of human hepatitis B virus (HBV) in stably transfected hepatoblastoma cells: A novel system for screening potential inhibitors of HBV replication
Inducible expression of human hepatitis B virus (HBV) in stably transfected hepatoblastoma cells: A novel system for screening potential inhibitors of HBV replication
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DOI：
10.1128/aac.41.8.1715
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发表时间：
1997-08-01
影响因子：
4.9
通讯作者：
King, RW
中科院分区：
文献类型：
--
作者：
Ladner, SK;Otto, MJ;King, RW
我们报告了一种名为 HepAD38 的细胞系的开发和分离，该细胞系在可用四环素调节的条件下复制人类乙型肝炎病毒 (HBV)。在抗生素存在的情况下，由于前基因组（pg）RNA合成的抑制，该细胞系不含病毒。从培养基中去除四环素后，细胞表达病毒pg RNA，在细胞质中积累亚病毒颗粒，其中含有病毒复制特征的DNA中间体，并将病毒样颗粒分泌到上清液中。由于 HepAD38 细胞系可以产生高水平的 HBV DNA，因此它对于分析依赖于同步方式的病毒 DNA 合成的病毒复制周期应该很有用。此外，该细胞系已被格式化为高通量、基于细胞的检测，允许大规模筛选各种化合物库以寻找新类别的 HBV 复制抑制剂。
We report the development and isolation of a cell line, termed HepAD38, that replicates human hepatitis B virus (HBV) under conditions that can be regulated with tetracycline. In the presence of the antibiotic, this cell line is free of virus due to the repression of pregenomic (pg) RNA synthesis, Upon removal of tetracycline from the culture medium, the cells express viral pg RNA, accumulate subviral particles in the cytoplasm that contain DNA intermediates characteristic of viral replication, and secrete virus-like particles Into the supernatant. Since the HepAD38 cell line can produce high levels of HBV DNA, it should be useful for analyses of the viral replication cycle that depend upon viral DNA synthesis in a synchronized fashion, In addition, this cell line has been formatted into a high-throughput, cell-based assay that permits the large-scale screening of diverse compound libraries for new classes of inhibitors of HBV replication.