The genetics of feeding in Caenorhabditis elegans.
The genetics of feeding in Caenorhabditis elegans.
复制标题
秀丽隐杆线虫的摄食遗传学。
DOI：
10.1093/genetics/133.4.897
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发表时间：
1993
期刊：
影响因子：
3.3
通讯作者：
Avery,L
中科院分区：
文献类型：
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作者：
Avery,L
秀丽隐杆线虫的咽部是一个几乎独立的神经肌肉器官，负责进食。为了鉴定与咽部可兴奋细胞发育或功能有关的基因，我筛选了咽部摄食行为有明显缺陷的蠕虫。52个突变确定了35个基因，其中至少22个是以前未知的。这些基因可分为三大类：2个pha基因，突变导致咽形状缺陷；7个phm基因，突变导致咽肌收缩结构缺陷；26个eat基因，突变导致咽肌运动异常，但咽形状正常，双折射正常，能够剧烈收缩。虽然Eat表型多样，但大多数类似于咽神经系统缺陷引起的表型。先前的基因镶嵌和药理学研究有直接证据表明，一些饮食基因确实影响神经系统。在eat-5突变体中，咽部不同部位的运动不太同步。Eat-6和eat-12突变体未能适当放松咽肌。这些咽运动缺陷最容易解释为咽肌膜异常的电兴奋性。
The pharynx of Caenorhabditis elegans is a nearly self-contained neuromuscular organ responsible for feeding. To identify genes involved in the development or function of the excitable cells of the pharynx, I screened for worms with visible defects in pharyngeal feeding behavior. Fifty-two mutations identified 35 genes, at least 22 previously unknown. The genes broke down into three broad classes: 2 pha genes, mutations in which caused defects in the shape of the pharynx, 7 phm genes, mutations in which caused defects in the contractile structures of the pharyngeal muscle, and 26 eat genes, mutants in which had abnormal pharyngeal muscle motions, but had normally shaped and normally birefringent pharynxes capable of vigorous contraction. Although the Eat phenotypes were diverse, most resembled those caused by defects in the pharyngeal nervous system. For some of the eat genes there is direct evidence from previous genetic mosaic and pharmacological studies that they do in fact affect nervous system. In eat-5 mutants the motions of the different parts of the pharynx were poorly synchronized. eat-6 and eat-12 mutants failed to relax their pharyngeal muscles properly. These pharyngeal motion defects are most easily explained as resulting from abnormal electrical excitability of the pharyngeal muscle membrane.